Flashcards in Protues-Providencia-Morganella Deck (85):
Different strains of proteus, when inoculated in culture medium swarms toward each other, but do not mingle thus leaving a demarcation line between them.
Actively motile at 37C, pleomorphic,NLF, urease(+), Phenlyalanine Deaminase(+); Rapidly hydrolyzes urease at 2-4hours. Surface growth that gives off a BURNT GUNPOWDER ODOR; Swarming motile on BAP
Pathogens of proteus
Wound and Urinary Tract Infection, nosocomial infection, bacteremia, pneumonia
Differenting point of P.mirabilis and P.vulgaris
Most important member of proteus genus. Susceptible to ampicillin, chloramphenicol, cephalosporin. OX-K(kingsburry); may cause pneumonia and septicemia. Indole (-). Tend to swarm on moist agar producing bluish gray confluent.
Targets the immunosuppressed individuals; OX-02 and OX-19; INDOLE (+); may cause nosocomial infections and uti. Resistant to Ampicillin, chloramphenicol, cephalosporin. Has the same antigenic structure as rickettsiae such that O antigen
To detect rickettsial infections.
Most important species of morganella. Causes UTI and wound infections as well as diarrhea. Lactose, citrate, h2s and LDC(-); urease and Deaminase (+). Formerly known as P.morganii
Normal intestinal flora; difficult to treat because most of them are R to antibiotics. Associated with nosocomial infection, gram -, NLF, pleomorphic, motile at 25 , non motile at 31c. Urease - and NLF
Associated with some cases of diarrhea in children. Infection are rare. Do not swarm on BAP.
Other Providencia species
PROVIDENCIA STUARTII, PROVIDENCIA RETTGERI, PROVIDENCIA RUTSIGANII.
NLF, microaerophilic, short coccobacilli, exhibits bipolar granules, ferments sucrose.
Yersinia on Desoxycholate agar
REDDISH PINK COLONIES; DOES NOT FERMENTS LACTOSE; AEROGENIC
Yersinia on old culture
ORGANISM FORMS SURFACE GROWTH PELLICLE WITH STALACTITE STREAMERS
Virulence factors of Yersinia.
ENDOTOXINS, COAGULASE, FIBRINOLYSIN
Two most important species of Yersinia, that causes enterocilitis.
Y.enterocolitica and Y.pestis
Causative agent of Y.pseudotuberculosis or Y.enterocolitis; can cause a severe intestinal inflammation
Opportunistic pathogens of Yersinia.
Y.intermedia, Y.freduikaenii, Y.kristensenii
Most common Yersinia isolated from human. Causes enterocolitis. Most often encountered Yersinia in the lab. Invasive pathogen which can penetrate gut lining and enter the lymphatic system and blood. Infections are usually through an ingestion of contaminated food, which can cause a severe intestinal inflammation. Release of it's enterotoxin cause a severe pain similar to that found in patients with appendicitis. Easily found because it has the ability to grown in a cold temp and motile at room temp.
Also known as plaque bacillus.
Infection of wild rodents transmitted occasionally by bite of fleas
From oriental rat flea bite. Symptoms niya is swelling of lymph nodes. Nagcacause ng cell death and black purpuric lesions or blak death.
3 human plague in Y.pestis. Lymph nodes. Buboes
3 human plague in Y.pestis. Airborne transmission
3 human plague in Y.pestis. Black Death due to Schwartzmann phenomenon.
Mesetric lymphadenitis, septicemia. LOA --, Urease +, ODC and sucrose -.
Stained smears of Y.pseudotuberculosis.
Wayson and Giema stain.
Culture of MAC on Y.pseudotuberculosis.
Colorless to peach
Culture of emb in y.pseudotuberculosis.
Colorless to purple
Culture of HEA on y.pseudotuberculosis.
Culture of XLD in Y.pseudotuberculosis
Culture of SSA on Y.pseudotuberculosis.
Transport medium for Y.pseudotuberculosis. For transporting and maintaining tissue infected with Y.pestis, also useful for transporting stool for isolation of shigella, salmonella and Yersinia.
Found in brackish or salt water. Short, facultative anaerobes, ferments glucose, NLF, reduce nitrates to nitrites, Oxidase + except V.metschnikovii, INDOLE +, catalase +, LOA ++-. Hemophilic organism except V.cholera and V.mimicus, natural habitat is water, usually associated with seafood or dairy. Motile, comma/curved bacillus. Not an enteric gastrointestinal pathogen but has the ability to cause gastroenteritis.
Members of Family Vibrionaceae.
Vibrio, Aermonas, Plesiomonas
Vibrio comma. Curve/comma shaped rods, gram - rods, facultative anaerobes, non halophilic ferments glucos
V.cholerae under contrast or dark field microscopy
Darting motility or shooting star motility
Potent enterotoxin on V.cholerae
Cholera toxin, zit toxin, ace toxin.
Cholera toxin in V.cholerae
Cholerogen 01 and 0139
String test of V.cholerae
Reagent: 0.5% sodium desoxycholate +; sucrose fermenters (yellow TCBS)
Transport medium of V.cholerae
Enrichment medium of V.cholerae
Alkaline Peptone Water
Culture of V.cholerae on BAP
Smooth, medium to large colonies with greenish blue
V.cholerae on TCBS
pH 8.4. Yellow colonies
Disease characterized by loss of large amounts of fluids
Toxin in V. Cholerae that is a rice watery stool
Toxin in V.cholerae that is found in chromosome
Causative agent of V. Cholera
V. Cholera eltor
Serological type: V. Cholerae 01 serotypes: found in the Phil.
Original J or Inaba type
Serological type: V. Cholerae 01 serotypes: found in Japan
Middle intermediate or Hikojjma type
Serological type: V. Cholerae 01 serotypes: in India
Variant F or Ogawa type
Lysis of v. Cholerae when inoculated into an immuned guinea pigs
Phenomenon of Bacteriolysis
Classical is - and Eltor +
Bio types: RBC hemolysis, VP, aggtn. With chicken RBC
Classical s and Eltor is r
Bio type polymixin B
Halophilic organisms causing gastroenteritis and food poisoning associated with consumption of a contaminated seafoods.
Factors causing hemolysin
Heat stable and labile direct hemolysin, phospholipase A and Lysophospholipase
Phenomenon of V. Parahaemolyticus
Ear and wound infection. Associated with marine environment, halophilic, sucrose +, yellow on TCBS.
Septecimia, wound infection involving marine environment. Known to cause necrotizing fasciitis, extremely virulent, green on TCBS. Associated with eating raw oyster.
Food poisoning, non marine vibrio, green on TCBS, non halophilic, sucrose -
Facultative anaerobes, oxidase and catalase +, motile but some species are not motile. Bull's eye colonies on CIN, beta-hemolytic on BAP, can grown on Mac, EMB, SSA, CIN. Isolated from tap water, soil, river, marine environment and various foods. Halophilic. Pathogens of cold blooded animals such as frogs and snakes.
Causes gastroenteritis and cellulitis
A. Sobra and A. Hyrdophilia
Water loving organism, associated with gastrointestinal disease
Characteristics that separated it from other member of Enterobacteriaceae. Oxidase +
Biochemically similar to shigella. Motile, oxidase +, do not produce gas. Can grow on media that used for enteric bacteria, found in fresh water especially in warmer climates. Glucose fermenters and genetically related to Proteus. Exposure to cold blooded animals such as reptiles and may cause gastritis.
Species used for P.shigelloides
Stool and rectal swab
Separates Vibrio spp. From Aeromonas sp and P.shigelloides
String test: 0.5% sodium desoxycholate
Differentiated V. Cholerae 01 and non 01 from other species
Vibrio static test- 0129
Small spirals, s shaped, sea gull winged forms, sensitive to h2o2 and superoxide, oxidase +, catalase +, urease -, no3 +, gram -, monotrichous flagella, non-CHO fermenters, unable to grow on 3.5% NaCl. Most common cause of gastroenteritis in the US. Also cause septicarthitis. Most recognized antecedent cause of gullain Barre syndrome.
Susceptible in nalidix acid, resistant in cephalosporin, able to hydrolyzes hippurate.
Optimal Incubator temp of c. Jejuni
Specimens of CampylobacterJejuni
Feces, rectal swab and blood
Other species of Campylobacter
C. Jejuni and C. Coli
Animal pathogen that causes animal abortion
Serolgy of campylobacter Jejuni: for soluble heat stable agglutination
Heat labile with slide agglutination tech. C. Laridis, C. Coli, C. Jejuni
Formerly vibrios fetus
Transport media for C.Fetus
Buffered saline glycerol and alkaline Peptone water.
Formerly identified as C.pylori, spiral shaped resembling Campylobacter . NS. Causes type B gastritis, peptic ulcer, gastric carcinoma, bad breath and cancer. Rodlike bizarre, u shaped gr(-) bacteria, tuft or polar flagella with corkscrew motility. Strongly urease and catalase +.
Specimens used for Helicobacter pylori
Urine and tissue biopsy material
Differentiating point with campylobacter and Helicobacter
Motility with 4-6 flagella and optimum temp 35-37C.