Protues-Providencia-Morganella Flashcards Preview

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Flashcards in Protues-Providencia-Morganella Deck (85):
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Different strains of proteus, when inoculated in culture medium swarms toward each other, but do not mingle thus leaving a demarcation line between them.

DIENESPHENOMENON

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Actively motile at 37C, pleomorphic,NLF, urease(+), Phenlyalanine Deaminase(+); Rapidly hydrolyzes urease at 2-4hours. Surface growth that gives off a BURNT GUNPOWDER ODOR; Swarming motile on BAP

PROTEUS

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Pathogens of proteus

Wound and Urinary Tract Infection, nosocomial infection, bacteremia, pneumonia

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Differenting point of P.mirabilis and P.vulgaris

INDOLE

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Most important member of proteus genus. Susceptible to ampicillin, chloramphenicol, cephalosporin. OX-K(kingsburry); may cause pneumonia and septicemia. Indole (-). Tend to swarm on moist agar producing bluish gray confluent.

PROTEUS MIRABILIS

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Targets the immunosuppressed individuals; OX-02 and OX-19; INDOLE (+); may cause nosocomial infections and uti. Resistant to Ampicillin, chloramphenicol, cephalosporin. Has the same antigenic structure as rickettsiae such that O antigen

PROTEUS VULGARIS

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To detect rickettsial infections.

WEIL-FELIX TEST

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Most important species of morganella. Causes UTI and wound infections as well as diarrhea. Lactose, citrate, h2s and LDC(-); urease and Deaminase (+). Formerly known as P.morganii

MORGANELLA MORGANII

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Normal intestinal flora; difficult to treat because most of them are R to antibiotics. Associated with nosocomial infection, gram -, NLF, pleomorphic, motile at 25 , non motile at 31c. Urease - and NLF

PROVIDENCIA

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Associated with some cases of diarrhea in children. Infection are rare. Do not swarm on BAP.

PROVIDENCIA ALCALIFACIENS

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Other Providencia species

PROVIDENCIA STUARTII, PROVIDENCIA RETTGERI, PROVIDENCIA RUTSIGANII.

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NLF, microaerophilic, short coccobacilli, exhibits bipolar granules, ferments sucrose.

YERSINIA

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Yersinia on Desoxycholate agar

REDDISH PINK COLONIES; DOES NOT FERMENTS LACTOSE; AEROGENIC

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Yersinia on old culture

ORGANISM FORMS SURFACE GROWTH PELLICLE WITH STALACTITE STREAMERS

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Virulence factors of Yersinia.

ENDOTOXINS, COAGULASE, FIBRINOLYSIN

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Two most important species of Yersinia, that causes enterocilitis.

Y.enterocolitica and Y.pestis

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Causative agent of Y.pseudotuberculosis or Y.enterocolitis; can cause a severe intestinal inflammation

Yersiniosis

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Opportunistic pathogens of Yersinia.

Y.intermedia, Y.freduikaenii, Y.kristensenii

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Most common Yersinia isolated from human. Causes enterocolitis. Most often encountered Yersinia in the lab. Invasive pathogen which can penetrate gut lining and enter the lymphatic system and blood. Infections are usually through an ingestion of contaminated food, which can cause a severe intestinal inflammation. Release of it's enterotoxin cause a severe pain similar to that found in patients with appendicitis. Easily found because it has the ability to grown in a cold temp and motile at room temp.

Y.enterocolitica

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Also known as plaque bacillus.

Y.pestis

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Infection of wild rodents transmitted occasionally by bite of fleas

Plague

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From oriental rat flea bite. Symptoms niya is swelling of lymph nodes. Nagcacause ng cell death and black purpuric lesions or blak death.

Xenopsylla cheopsis

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3 human plague in Y.pestis. Lymph nodes. Buboes

Bubonic plague

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3 human plague in Y.pestis. Airborne transmission

Pnuemonic plague

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3 human plague in Y.pestis. Black Death due to Schwartzmann phenomenon.

Septicemic plague

25

Mesetric lymphadenitis, septicemia. LOA --, Urease +, ODC and sucrose -.

Y.psuedotuberculosis

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Stained smears of Y.pseudotuberculosis.

Wayson and Giema stain.

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Culture of MAC on Y.pseudotuberculosis.

Colorless to peach

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Culture of emb in y.pseudotuberculosis.

Colorless to purple

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Culture of HEA on y.pseudotuberculosis.

Salmon

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Culture of XLD in Y.pseudotuberculosis

Yellow

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Culture of SSA on Y.pseudotuberculosis.

Purple

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Transport medium for Y.pseudotuberculosis. For transporting and maintaining tissue infected with Y.pestis, also useful for transporting stool for isolation of shigella, salmonella and Yersinia.

Cary-Blaire medium

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Found in brackish or salt water. Short, facultative anaerobes, ferments glucose, NLF, reduce nitrates to nitrites, Oxidase + except V.metschnikovii, INDOLE +, catalase +, LOA ++-. Hemophilic organism except V.cholera and V.mimicus, natural habitat is water, usually associated with seafood or dairy. Motile, comma/curved bacillus. Not an enteric gastrointestinal pathogen but has the ability to cause gastroenteritis.

Family Vibrionaceae

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Members of Family Vibrionaceae.

Vibrio, Aermonas, Plesiomonas

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Vibrio comma. Curve/comma shaped rods, gram - rods, facultative anaerobes, non halophilic ferments glucos

Vibrio cholerae

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V.cholerae under contrast or dark field microscopy

Darting motility or shooting star motility

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Potent enterotoxin on V.cholerae

Cholera toxin, zit toxin, ace toxin.

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Cholera toxin in V.cholerae

Cholerogen 01 and 0139

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String test of V.cholerae

Reagent: 0.5% sodium desoxycholate +; sucrose fermenters (yellow TCBS)

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Transport medium of V.cholerae

Amies medium

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Enrichment medium of V.cholerae

Alkaline Peptone Water

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Culture of V.cholerae on BAP

Smooth, medium to large colonies with greenish blue

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V.cholerae on TCBS

pH 8.4. Yellow colonies

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Disease characterized by loss of large amounts of fluids

Asiatic Cholera

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Toxin in V. Cholerae that is a rice watery stool

Endotoxins

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Toxin in V.cholerae that is found in chromosome

Enterotoxin

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Causative agent of V. Cholera

V. Cholera eltor

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Serological type: V. Cholerae 01 serotypes: found in the Phil.

Original J or Inaba type

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Serological type: V. Cholerae 01 serotypes: found in Japan

Middle intermediate or Hikojjma type

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Serological type: V. Cholerae 01 serotypes: in India

Variant F or Ogawa type

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Lysis of v. Cholerae when inoculated into an immuned guinea pigs

Bacteriolysis

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Phenomenon of Bacteriolysis

Pfeiffer's Phenomenon

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Classical is - and Eltor +

Bio types: RBC hemolysis, VP, aggtn. With chicken RBC

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Classical s and Eltor is r

Bio type polymixin B

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Halophilic organisms causing gastroenteritis and food poisoning associated with consumption of a contaminated seafoods.

Vibrio Parahaemolyticus

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Factors causing hemolysin

Heat stable and labile direct hemolysin, phospholipase A and Lysophospholipase

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Phenomenon of V. Parahaemolyticus

Kaganawa Phenomenon

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Ear and wound infection. Associated with marine environment, halophilic, sucrose +, yellow on TCBS.

V. Aglinolyticus

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Septecimia, wound infection involving marine environment. Known to cause necrotizing fasciitis, extremely virulent, green on TCBS. Associated with eating raw oyster.

V. Vulnificus

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Wound infection

V. Damsela

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Food poisoning

V. Fluvialis

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Food poisoning, non marine vibrio, green on TCBS, non halophilic, sucrose -

V. Mimicus

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Facultative anaerobes, oxidase and catalase +, motile but some species are not motile. Bull's eye colonies on CIN, beta-hemolytic on BAP, can grown on Mac, EMB, SSA, CIN. Isolated from tap water, soil, river, marine environment and various foods. Halophilic. Pathogens of cold blooded animals such as frogs and snakes.

Aeromonas

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Causes gastroenteritis and cellulitis

A. Sobra and A. Hyrdophilia

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Water loving organism, associated with gastrointestinal disease

Aeromonas hydrophilia

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Characteristics that separated it from other member of Enterobacteriaceae. Oxidase +

Plesiomonas

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Biochemically similar to shigella. Motile, oxidase +, do not produce gas. Can grow on media that used for enteric bacteria, found in fresh water especially in warmer climates. Glucose fermenters and genetically related to Proteus. Exposure to cold blooded animals such as reptiles and may cause gastritis.

Plesiomonas Shigelloides

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Species used for P.shigelloides

Stool and rectal swab

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Separates Vibrio spp. From Aeromonas sp and P.shigelloides

String test: 0.5% sodium desoxycholate

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Differentiated V. Cholerae 01 and non 01 from other species

Vibrio static test- 0129

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Small spirals, s shaped, sea gull winged forms, sensitive to h2o2 and superoxide, oxidase +, catalase +, urease -, no3 +, gram -, monotrichous flagella, non-CHO fermenters, unable to grow on 3.5% NaCl. Most common cause of gastroenteritis in the US. Also cause septicarthitis. Most recognized antecedent cause of gullain Barre syndrome.

Campylobacter Jejuni

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Susceptible in nalidix acid, resistant in cephalosporin, able to hydrolyzes hippurate.

Camplyobacter Jejuni

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Optimal Incubator temp of c. Jejuni

42C

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Specimens of CampylobacterJejuni

Feces, rectal swab and blood

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Other species of Campylobacter

C. Jejuni and C. Coli

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Animal pathogen that causes animal abortion

C. Lari

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Serolgy of campylobacter Jejuni: for soluble heat stable agglutination

Penner method

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Heat labile with slide agglutination tech. C. Laridis, C. Coli, C. Jejuni

Lior Method

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Formerly vibrios fetus

Campylobacter Fetus

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Transport media for C.Fetus

Buffered saline glycerol and alkaline Peptone water.

81

Formerly identified as C.pylori, spiral shaped resembling Campylobacter . NS. Causes type B gastritis, peptic ulcer, gastric carcinoma, bad breath and cancer. Rodlike bizarre, u shaped gr(-) bacteria, tuft or polar flagella with corkscrew motility. Strongly urease and catalase +.

Helicobacter pylori

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Specimens used for Helicobacter pylori

Urine and tissue biopsy material

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Differentiating point with campylobacter and Helicobacter

Motility with 4-6 flagella and optimum temp 35-37C.

84

Excellent sensitivity and specificity lab diagnosis of Helicobacter pylori

Urea breath test