papillomaviruses and retroviruses

Question 1

Pervasive. Nearly everyone will be infected with an HPV at some point in their lifetime

Answer 1

papillomavirus

Question 2

non-enveloped, icosahedral with a 8kb, circular dsDNA

Answer 2

Papillomavirus

Question 3

papillomaviruses initially attach to ________ on the basement membrane

Answer 3

heparan sulfate proteoglycans

Question 4

papillomaviruses infections begin in

Answer 4

basal layer

Question 5

papillomaviruses enter by

Answer 5

receptor-mediated endocytosis

Question 6

papillomavirus is trafficked to late endosomes, similar to picornaviruses but

Answer 6

caspid disassembles in the endosome

Question 7

gene expression is tightly coupled to differentiation state of

Answer 7

epithelial cells

Question 8

is the master regulator of viral gene expression by directing synthesis of other early mRNAs including E6 and E7

Answer 8

E2

Question 9

early synthesis of _____ and ____ mRNA using cell-specific promoter and differential splicing

Answer 9

E1 and E2

Question 10

binds p53, preventing cell death by apoptosis

Answer 10

E6

Question 11

binds Rb and other proteins stimulating entry into the cell cycle

Answer 11

E7

Question 12

directs initiation of DNA replication

Answer 12

E2

Question 13

viral genome is mantained at a

Answer 13

high copy number

Question 14

late gene expression occurs only in terminally differentiated

Answer 14

keratinocytes

Question 15

keratinocyte differentiation activates late promoter and

Answer 15

stimulates DNA replication

Question 16

differential splicing to give mRNAs for

Answer 16

L1 and L2 capsid proteins

Question 17

Transport of L1 and L2 proteins to nucleus for

Answer 17

assembly into caspid

Question 18

virions do not __________, they are released by normal ketatinocyte death and loss membrane integrity

Answer 18

lyse the cell

Question 19

those that will rise to a cancer will have E6 and E7 but not

Answer 19

E2

Question 20

replicate through a DNA intermediate (provirus) integrated into the host genome

Answer 20

retroviruses

Question 21

enveloped single stranded (+) RNA, 1-10 kb

Answer 21

retrovirus

Question 22

____ polyprotein forms virion. structural protein

Answer 22

Gag

Question 23

retrovirus embedded in viral membrane ; glycoprotein

Answer 23

Env

Question 24

viral enzymes: protease, reverse transcriptase and integrase

Answer 24

pol

Question 25

in a retrovirus the virion must contains the viral enzyme

Answer 25

RT PR IN

Question 26

reverse transcription in a retrovirus occurs

Answer 26

immediately upon entry

Question 27

in retroviruses, DNA form of the genome is trafficked to the nucleus, where

Answer 27

the entire genome is integrated into the host genome by the viral integrase

Question 28

synthesis of (-) DNA to 5-end using host

Answer 28

tRNA primer

Question 29

finish product in reverse transcription

Answer 29

double DNA provirus integrated into genome

Question 30

in reverse trancription, difference btween RNA genome and DNA genome

Answer 30

ends are different in RNA but in DNA it is the same

Question 31

retrovirus DNA integration carried out by

Answer 31

viral integrase

Question 32

simple retrovirus require

Answer 32

cell division

Question 33

complex retrovirus (HIV) traffic

Answer 33

mRNAs out to ctoplasm

Question 34

mRNA requires splicing

Answer 34

Env

Question 35

unspliced mRNA is used to express

Answer 35

Gag and Pol

Question 36

new virion is not infectious until it

Answer 36

matures

Question 37

maturation step in which these polyprotein are cleaved int their constituent proteins by viral peptidases

Answer 37

Gag and Pro

Question 38

1 mechanism of transformation

Answer 38

proviral DNA integrates into the genome upstream of a proto-oncogene--> LTR can deregulate the Proto-oncogene and forming it into an oncogene

Question 39

2 mechanism of transformation

Answer 39

retrovirus contain the proto-oncogene in its genome; any cell infected will become cancerous

Question 40

Identify between retrovirus and papillomavirus: 1. integration is part of a normal replication cycle 2. integration is a rare chance event and not part of normal virus life cycle

Answer 40

1. retrovirus | 2. papillomavirus

Question 41

Identify between retrovirus and papillomavirus: 1. oncogene product interacts with members of the pathway that stimulates cells into S-phase DNA synthesis 2. Many of the same oncogenes are altered by mutation in non-viral human cancers

Answer 41

1. papillomaviruses | 2. retroviruses

Question 42

Identify between retrovirus and papillomavirus: 1. oncogenes are viral genes 2. oncogene can be either in virus or cell genome

Answer 42

1. papillomavirus | 2. retroviruses