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Flashcards in PH166 intro/Analytical Components Deck (48):
1

List grades of water @ clin lab

Feedwater/tap water
Clinical laboratory reagent water

2

Clinical laboratory reagent water - how is it prepared? What methods?

Filtration
Ion exchange
Carbon adsorption chromatography
UV light treatment

3

quality control and impurity testing procedures used for diff grades of reagent water - enumerate

Microbial monitoring
Resistivity measurements
Silica measurements
Biannual decontamination

4

Differentiate between a primary standard and Standard Reference Material

SRMs are preparation of stated composition that may be used as primary standards

5

Differentiate bet "to contain" & "to deliver"

"To contain" - to hold but not transfer
"To deliver" - transfer

6

Explain difference bet volumetric pipets and graduated pipets

Volumetric - designed to deliver a fixed volume without further subdivisions

Graduated - capable of dispensing several diff volumes

7

Safety regulations of the OSHA require two things: enumerate

Chemical Hygiene Plan
Fire Safety Plan

8

Define MSDS and explain its use

Contains info abt physical & health hazards for all chemical, reagents and kits used by laboratory

9

Meaning of National Fire Protection Agency blue diamond?

Health hazard

10

Define a desiccant and explain how it is used

Desiccant are chemicals that usually are found in a solid form & can absorb water. It may be used to dry and keep dry another chemical

11

Grades of Chemicals used: enumerate, and rank accdg to quality

1. Analytical grade
2. Chemically pure grade
3. US Pharmacopeia/National Formulary
4. Technical/Commercial Grade

12

highly purified chemical that can be measured directly to produce a substance of exact known concentration and purity

Primary Standard

13

T/F
Not all standar reference materials are as pure as primary standards

T

14

Organic solvents that are spectrograde/nanograde/HPLC Grade must meet some criteria. enumerate :)

1. purity ensures minimal spectral interference and min residual conc
2. >99% pure. no single impurity exceeding 0.2%

15

What is cobalt chloride's role in a desiccant?

moisture-sensitive indicator salt present in most desiccants. indicates exhaustion

16

False about anhydrous calcium chloride
A. high capacity
B. works well in

C

17

True for both anhydrous MgSO4 & anhydrous CaSO4
A. inexpensive
B. fast acting
C. both A and B
D. neither A nor B

B

18

Why can't Type I reagent water be absorbed for a long time?

it may absorb carbon dioxide

19

used in preparing buffers and standard solutions
A. Type I Reagent Water
B. Type II Reagent Water
C. Type III Reagent Water

A

20

used in quali chem, hema, microbio procedures
A. Type I Reagent Water
B. Type II Reagent Water
C. Type III Reagent Water

B

21

used in general urinalysis
A. Type I Reagent Water
B. Type II Reagent Water
C. Type III Reagent Water

C

22

T/F
Sterile water is always reagent water in hospitals

F
not always

23

T/F
As water purity increases, water conductivity increases.

F
water purity--> less dissolved ionized substance --> less conductivity --> more resistance

24

False about distillation
A. removes volatile impurities
B. meets specs for type II & III water
C. not useful for elimination of low-boiling org cpd
D. removes Fe, Mg, Ca + microbes

A

25

False about deionization
A. water passed thru resin column
B. (+) and (-) charged particles in column combine with ions present to in water to remove them
C. substances that don't ionize are removed

C
They are not removed

26

A deionization process is source of type I water if further treated with __ & __

membrane filtration
activated charcoal

27

False about water purification?
A. ozone: used in industrial setting
B. reverse osmosis: removes dissolved gases effectively
C. UV oxidation- removes bacteria and org cpds

B
it does not remove such gases effectively

28

Which is false about tubing?
A. latex rubber tubing: not affected by oils
B. neoprene: not used with chlorinated HCs
C. Tygon tubing:inert to chemicals
D. Teflon tubing: more expensive than Tygon

A

29

Pyrex and Kimax: type of __ glass

borosilicate

30

glassware: ideal for ___ solution
(Acidic, alkaline)

acidic

31

plasticware: ideal for ___ solution
(Acidic, alkaline)

alkaline

32

Which is more apt for use @ high thermal experiments?
A. glassware
B. plastic ware
C. either

A

33

Which is more apt for use @ cryogenic experiments?
A. glassware
B. plastic ware
C. either

B

34

Differentiate Mohr pipet fr Serological pipet

Mohr - calibrated between two marks of the stem. may space after ng dulo ng marks
Serological - has graduation marks down on the tip

35

pipet with bulb closer to delivery tip. used for measuring viscous fluid in blood/serum

identify

Ostwald-Folin pipet

36

Mohr pipet or Serological pipet
Which has larger orifice?

Serological pipet

37

Which micropipets may also be used to deliver substances?

Lang-Levy
Sanz

38

Conditions in cleaning glassware & reagent
which is mismatched?
A. blood clot: 2-4% cresol solution
B. metal ion det: 20% nitric acid
C. new pipets: 5% HCl

A
10% NaOH dapat

39

Conditions in cleaning glassware & reagent
which is mismatched?
A. bacteriologic glassware: 2-4% cresol solution
B. iron determination: 1:3 nitric acid
C. permanganate stains: 5% HCl

C
50% dapat

40

differentiate flammable fr combustible liquid

flammable may form ignitible mixture @ 37.8 deg C

41

T/F
Non-ionizing radiation equipment often emits a variety of wavelengths of EM radiation that must be protected against, with the use of PPE

T

42

In decontamination, identify 2 common solutions used

sodium hypochlorite
70% alcohol

43

Meaning of National Fire Protection Agency red diamond?

flammability

44

Meaning of National Fire Protection Agency yellow diamond?

reactivity warning

45

Meaning of National Fire Protection Agency white diamond?

special hazard info

46

Enumerate elements of Hazard Warning System @ reagent bottle.

1- identify hazardous chemical
2- route of body entry
3- health hazard
4- physical hazard
5- target organ affected

47

Which is falsE?
A. solvents such as xylene and acetone may not be redistilled anymore
B. flammables can be incinerated with afterburners & scrubbers to remove toxic products of combustion
C. both A and B
D. NOTA

A

48

Enumerate approved methods for treating biomedical waste.

1- incinerator
2- steam sterilization
3- chemical disinfection
4- encapsulation @ solid matrix