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PH166 intro/Analytical Components Flashcards

1
Q

List grades of water @ clin lab

A

Feedwater/tap water

Clinical laboratory reagent water

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2
Q

Clinical laboratory reagent water - how is it prepared? What methods?

A

Filtration
Ion exchange
Carbon adsorption chromatography
UV light treatment

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3
Q

quality control and impurity testing procedures used for diff grades of reagent water - enumerate

A

Microbial monitoring
Resistivity measurements
Silica measurements
Biannual decontamination

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4
Q

Differentiate between a primary standard and Standard Reference Material

A

SRMs are preparation of stated composition that may be used as primary standards

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5
Q

Differentiate bet “to contain” & “to deliver”

A

“To contain” - to hold but not transfer

“To deliver” - transfer

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6
Q

Explain difference bet volumetric pipets and graduated pipets

A

Volumetric - designed to deliver a fixed volume without further subdivisions

Graduated - capable of dispensing several diff volumes

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7
Q

Safety regulations of the OSHA require two things: enumerate

A

Chemical Hygiene Plan

Fire Safety Plan

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8
Q

Define MSDS and explain its use

A

Contains info abt physical & health hazards for all chemical, reagents and kits used by laboratory

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9
Q

Meaning of National Fire Protection Agency blue diamond?

A

Health hazard

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10
Q

Define a desiccant and explain how it is used

A

Desiccant are chemicals that usually are found in a solid form & can absorb water. It may be used to dry and keep dry another chemical

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11
Q

Grades of Chemicals used: enumerate, and rank accdg to quality

A
  1. Analytical grade
  2. Chemically pure grade
  3. US Pharmacopeia/National Formulary
  4. Technical/Commercial Grade
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12
Q

highly purified chemical that can be measured directly to produce a substance of exact known concentration and purity

A

Primary Standard

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13
Q

T/F

Not all standar reference materials are as pure as primary standards

A

T

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14
Q

Organic solvents that are spectrograde/nanograde/HPLC Grade must meet some criteria. enumerate :)

A
  1. purity ensures minimal spectral interference and min residual conc
  2. > 99% pure. no single impurity exceeding 0.2%
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15
Q

What is cobalt chloride’s role in a desiccant?

A

moisture-sensitive indicator salt present in most desiccants. indicates exhaustion

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16
Q

False about anhydrous calcium chloride
A. high capacity
B. works well in

A

C

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17
Q 
True for both anhydrous MgSO4 & anhydrous CaSO4
A. inexpensive
B. fast acting
C. both A and B
D. neither A nor B
A

B

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18
Q

Why can’t Type I reagent water be absorbed for a long time?

A

it may absorb carbon dioxide

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19
Q

used in preparing buffers and standard solutions
A. Type I Reagent Water
B. Type II Reagent Water
C. Type III Reagent Water

A

A

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20
Q

used in quali chem, hema, microbio procedures
A. Type I Reagent Water
B. Type II Reagent Water
C. Type III Reagent Water

21
Q

used in general urinalysis
A. Type I Reagent Water
B. Type II Reagent Water
C. Type III Reagent Water

22
Q

T/F

Sterile water is always reagent water in hospitals

A

F

not always

23
Q

T/F

As water purity increases, water conductivity increases.

A

F

water purity–> less dissolved ionized substance –> less conductivity –> more resistance

24
Q

False about distillation
A. removes volatile impurities
B. meets specs for type II & III water
C. not useful for elimination of low-boiling org cpd
D. removes Fe, Mg, Ca + microbes

25
False about deionization A. water passed thru resin column B. (+) and (-) charged particles in column combine with ions present to in water to remove them C. substances that don't ionize are removed
C | They are not removed
26
A deionization process is source of type I water if further treated with __ & __
membrane filtration | activated charcoal
27
False about water purification? A. ozone: used in industrial setting B. reverse osmosis: removes dissolved gases effectively C. UV oxidation- removes bacteria and org cpds
B | it does not remove such gases effectively
28
Which is false about tubing? A. latex rubber tubing: not affected by oils B. neoprene: not used with chlorinated HCs C. Tygon tubing:inert to chemicals D. Teflon tubing: more expensive than Tygon
A
29
Pyrex and Kimax: type of __ glass
borosilicate
30
glassware: ideal for ___ solution | Acidic, alkaline
acidic
31
plasticware: ideal for ___ solution | Acidic, alkaline
alkaline
32
Which is more apt for use @ high thermal experiments? A. glassware B. plastic ware C. either
A
33
Which is more apt for use @ cryogenic experiments? A. glassware B. plastic ware C. either
B
34
Differentiate Mohr pipet fr Serological pipet
Mohr - calibrated between two marks of the stem. may space after ng dulo ng marks Serological - has graduation marks down on the tip
35
pipet with bulb closer to delivery tip. used for measuring viscous fluid in blood/serum identify
Ostwald-Folin pipet
36
Mohr pipet or Serological pipet | Which has larger orifice?
Serological pipet
37
Which micropipets may also be used to deliver substances?
Lang-Levy | Sanz
38
``` Conditions in cleaning glassware & reagent which is mismatched? A. blood clot: 2-4% cresol solution B. metal ion det: 20% nitric acid C. new pipets: 5% HCl ```
A | 10% NaOH dapat
39
Conditions in cleaning glassware & reagent which is mismatched? A. bacteriologic glassware: 2-4% cresol solution B. iron determination: 1:3 nitric acid C. permanganate stains: 5% HCl
C | 50% dapat
40
differentiate flammable fr combustible liquid
flammable may form ignitible mixture @ 37.8 deg C
41
T/F Non-ionizing radiation equipment often emits a variety of wavelengths of EM radiation that must be protected against, with the use of PPE
T
42
In decontamination, identify 2 common solutions used
sodium hypochlorite | 70% alcohol
43
Meaning of National Fire Protection Agency red diamond?
flammability
44
Meaning of National Fire Protection Agency yellow diamond?
reactivity warning
45
Meaning of National Fire Protection Agency white diamond?
special hazard info
46
Enumerate elements of Hazard Warning System @ reagent bottle.
``` 1- identify hazardous chemical 2- route of body entry 3- health hazard 4- physical hazard 5- target organ affected ```
47
Which is falsE? A. solvents such as xylene and acetone may not be redistilled anymore B. flammables can be incinerated with afterburners & scrubbers to remove toxic products of combustion C. both A and B D. NOTA
A
48
Enumerate approved methods for treating biomedical waste.
1- incinerator 2- steam sterilization 3- chemical disinfection 4- encapsulation @ solid matrix

PH 166 (28 decks)

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