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Flashcards in PH166 intro/Analytical Components Deck (48)
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1

List grades of water @ clin lab

Feedwater/tap water
Clinical laboratory reagent water

2

Clinical laboratory reagent water - how is it prepared? What methods?

Filtration
Ion exchange
Carbon adsorption chromatography
UV light treatment

3

quality control and impurity testing procedures used for diff grades of reagent water - enumerate

Microbial monitoring
Resistivity measurements
Silica measurements
Biannual decontamination

4

Differentiate between a primary standard and Standard Reference Material

SRMs are preparation of stated composition that may be used as primary standards

5

Differentiate bet "to contain" & "to deliver"

"To contain" - to hold but not transfer
"To deliver" - transfer

6

Explain difference bet volumetric pipets and graduated pipets

Volumetric - designed to deliver a fixed volume without further subdivisions

Graduated - capable of dispensing several diff volumes

7

Safety regulations of the OSHA require two things: enumerate

Chemical Hygiene Plan
Fire Safety Plan

8

Define MSDS and explain its use

Contains info abt physical & health hazards for all chemical, reagents and kits used by laboratory

9

Meaning of National Fire Protection Agency blue diamond?

Health hazard

10

Define a desiccant and explain how it is used

Desiccant are chemicals that usually are found in a solid form & can absorb water. It may be used to dry and keep dry another chemical

11

Grades of Chemicals used: enumerate, and rank accdg to quality

1. Analytical grade
2. Chemically pure grade
3. US Pharmacopeia/National Formulary
4. Technical/Commercial Grade

12

highly purified chemical that can be measured directly to produce a substance of exact known concentration and purity

Primary Standard

13

T/F
Not all standar reference materials are as pure as primary standards

T

14

Organic solvents that are spectrograde/nanograde/HPLC Grade must meet some criteria. enumerate :)

1. purity ensures minimal spectral interference and min residual conc
2. >99% pure. no single impurity exceeding 0.2%

15

What is cobalt chloride's role in a desiccant?

moisture-sensitive indicator salt present in most desiccants. indicates exhaustion

16

False about anhydrous calcium chloride
A. high capacity
B. works well in

C

17

True for both anhydrous MgSO4 & anhydrous CaSO4
A. inexpensive
B. fast acting
C. both A and B
D. neither A nor B

B

18

Why can't Type I reagent water be absorbed for a long time?

it may absorb carbon dioxide

19

used in preparing buffers and standard solutions
A. Type I Reagent Water
B. Type II Reagent Water
C. Type III Reagent Water

A

20

used in quali chem, hema, microbio procedures
A. Type I Reagent Water
B. Type II Reagent Water
C. Type III Reagent Water

B

21

used in general urinalysis
A. Type I Reagent Water
B. Type II Reagent Water
C. Type III Reagent Water

C

22

T/F
Sterile water is always reagent water in hospitals

F
not always

23

T/F
As water purity increases, water conductivity increases.

F
water purity--> less dissolved ionized substance --> less conductivity --> more resistance

24

False about distillation
A. removes volatile impurities
B. meets specs for type II & III water
C. not useful for elimination of low-boiling org cpd
D. removes Fe, Mg, Ca + microbes

A

25

False about deionization
A. water passed thru resin column
B. (+) and (-) charged particles in column combine with ions present to in water to remove them
C. substances that don't ionize are removed

C
They are not removed

26

A deionization process is source of type I water if further treated with __ & __

membrane filtration
activated charcoal

27

False about water purification?
A. ozone: used in industrial setting
B. reverse osmosis: removes dissolved gases effectively
C. UV oxidation- removes bacteria and org cpds

B
it does not remove such gases effectively

28

Which is false about tubing?
A. latex rubber tubing: not affected by oils
B. neoprene: not used with chlorinated HCs
C. Tygon tubing:inert to chemicals
D. Teflon tubing: more expensive than Tygon

A

29

Pyrex and Kimax: type of __ glass

borosilicate

30

glassware: ideal for ___ solution
(Acidic, alkaline)

acidic