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PH 166 > Specimen Collection &Handling > Flashcards

Specimen Collection &Handling Flashcards

1
Q

Which may directly affect hormone level in blood?

A

Tourniquet application

Diurnal variation

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2
Q

Change in posture may elevate __&__levels

A

Albumin

Calcium

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3
Q

Which may be affected by stress? __ increased & __ decreased

A

Total cholesterol

HDL cholesterol

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4
Q

What indicators may be increased due to fasting for both sexes after 48 hr?

A

Serum bilirubin

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5
Q

What indicators may be decreased due to fasting in women, after 72 hr?

A

Plasma glucose

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6
Q

Tourniquet application –> increased enzyme and protein bc __

A

Hemoconcentration

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7
Q

Most serum constituents remain constant in adults until __ in women & __in men

A

Menopause

Middle age

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8
Q

Which age group has low glycogen reserve?
A. Infant
B. Adult
C. Same

A

A

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9
Q

Enumerate physio factors that may contribute to result variation

A 
Stress
Diurnal Variation
Diet
Exercise
Age
Torniquet Application
Posture
Gender
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10
Q 
Tobacco smoking is an in-vivo interference to the lab result. It may elevate all except:
A. Hb conc
B. HDL
C. MCV
D. RBC count
E. WBC Count
A

B

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11
Q 
When the needle is \_\_\_, hemolysis may occur
A. too small
B. too big
C. either A or B
D. neither A nor B
A

A

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12
Q

Serum bilirubin > 430 mmol/L is called ___

A

icteric serum

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13
Q 
icteric serum: interference for assays of all except
A. albumin
B. total protein
C. cholesterol
D. bilirubin
A

D

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14
Q 
lipemic serum: interference for assays of all except
A. urea
B. total protein
C. cholesterol
D. bilirubin
A

C

amylase dapat

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15
Q

Arterial puncture: used for all except
A. complete blood count
B. pH measurement
C. blood gas analysis

A

A

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16
Q 
Unacceptable sites for arterial puncture include all except
A. inflammation site
B. site near a wound
C. site with AV fistula
D. site with deep arteries
A

D

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17
Q

identify most common preanalytic error in blood gas measurement

A

use of too much heparin

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18
Q 
all are common sites for venipuncture except
A. antecubital fossa
B. wrist and ankle
C. hand dorsum
D. jugular vein
A

D

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19
Q

antecubital fossa includes these 3 veins. enumerate

A

median cubital vein
cephalic vein
basilic vein

20
Q

purpose of Heel Skin/Finger Puncture

A

routine assays requiring small amounts of blood

21
Q

order of draw: evacuated tube and syringe

A
  1. yellow
  2. blue
  3. serum tubes with/without clot activ/gel sep
  4. green
  5. lavender
  6. silver
22
Q 
additive || color top
Which is matched properly?
A. EDTA || red
B. sodium heparin || gray
C. sodium fluoride || lavender
D. sodium citrate || blue
A

D

A. no additive || red
B. sodium heparin || green
C. sodium fluoride || gray

23
Q 
additive || color top
Which is matched properly?
A. EDTA || lavender
B. SST || blue
C. sodium heparin || gold
D. sodium citrate || yellow
A

A

B. SST || gold
C. sodium heparin || green
D. sodium citrate || blue

24
Q 
additive || color top
Which is matched properly?
A. SST || silver
B. sodium fluoride || gold
C. sodium heparin || green
D. sodium citrate || lavender
A

C

A. SST || gold
B. sodium fluoride || gray
D. sodium citrate || blue

25
concentration of EDTA @ tube
1-2 mg/mL of blood
26
lavender EDTA tube: purpose
hematology cell count and cell morphology
27
pink EDTA tube: purpose
ABO grouping, Rh typing, antibody screening
28
white EDTA tube: purpose
molecular diagnostic testing for plasma
29
sodium citrate black tube: purpose
Westergren sedimentation rate
30
sodium citrate light blue tube:purpose
coagulation testing
31
sodium citrate : concentation @ tube
3.2-3.8 g/dL of blood
32
``` true about heparin A. used in hematology testing B. affect ion levels, like calcium C. can interfere with some immunoassays D. NOTA ```
C
33
Differentiate lithium heparin fr sodium heparin
LiHep - used for most chemistry tests except lithium and folate NaHep - anticoagulant therapy, trace elements, toxicology
34
``` sodium fluoride: gray top. Which is false? A. used for measurement of heavy metals B. interferes with Na, K C. forms weakly dissoc Ca cpds D. NOTA ```
A glucose dapat
35
sodium fluoride : concentation @ tube
10 mg/mL of blood
36
oxalate : concentation @ tube
1-2 mg/mL of blood
37
Why can't oxalate be used in hematocrit?
It may shrink red cells
38
What may be in SSTs? Enumerate
clot activator | gel separator
39
red tube: no additive. three purposes?
blood bank immunology assays blood culture
40
enumerate 5 process that affect concentration of a blood constituent in the specimen during storage
1. adsorption to glass or plastic tubes 2. protein denaturation 3. evaporation of volatile compounds 4. water mvmt into cells 5. cont metab activities of RBC, WBC
41
``` These specimens must be kept at 4 deg C immediately after collection and transported on ice. Applicable to the ff except A. NH3 B. plasma renin C. acid phosphatase D. glucose ```
D
42
Serum required for the ff except A. protein electrophoresis B. coagulation measurements C. immunofixation assay
B
43
adequate time for clotting must be allowed before centrifugation. why?
prevent latent fibrin formation, which may cause undesirable clogging of automated chemistry analyzers
44
Three possible events in blood sample that may affect spectrophotometric methods of analysis. enumerate
hemolysis icterus lipemia
45
icterus may affect spectrophotometric method result because of ___, which has high absorbance at 340-500 nm
bilirubin
46
refers to the exclusion of water and small molecules in the aqueous phase, when more volume within a specimen is occupied by protein or lipid that excludes water
Solvent Exclusion Effect

PH 166 (28 decks)

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