Practical

Question 1

Answer 1

Aspergiullus

Question 2

Answer 2

Coccidioides

Question 3

Answer 3

Rhizopus

Question 4

Answer 4

penicillium

Question 5

Answer 5

baker’s yeast sexual

Saccharomyces cerevisiae

Question 6

What is the stain used in lab for molds

Answer 6

Lactophenol cotton Blue

Question 7

Domain and Kingdom of Yeast molds

Answer 7

Fungi and Eukaryotic

Question 8

Name of the selective medium for molds?

Answer 8

Sabouraud agar

Has a low pH and high sugar centration

Question 9

mycoses

Answer 9

An opportunistic disease when a host in immucompromised from a fungi.

Question 10

Endospore Stain Types/Example

Answer 10

1) Crystal violet method negative stain for enospores
2) Schaeffer-Fulton Method -endospores-green

Question 11

Acid-fast Examples/Technique

Answer 11

Ziehl Neelsen techique

Acid Fast - Pink - Mycobacterium smegmatis

Non Acid Fast - Blue- staphylococcus epidermidis

Question 12

Stain Capsule Technique/example

Answer 12

Capsuel Stain/Special Strain(India Ink)

Negative stain for capsule

Capsule - clear

Cells - red

Klebsialla pneumoniae

Question 13

Flagella

Answer 13

Leifson’s technique/ Flagella Stain

Specal stain

Mordant adheres to flagella

rolling drop slide

Yellow flagella

EX. Proteus vulgaris

Question 14

All rods are motile except

Answer 14

Klebsilla pnemoniae

Question 15

All Coccus are non motille except

Answer 15

Sporosarcina Ureae

Question 16

Answer 16

Plasmodial Slime Mold

Question 17

Answer 17

Crystal Violet

Endospore

Endospores colorless but highligheted in purple

Question 18

Answer 18

Schaeffer Fulton method/ endospore

endorspores stained malachite green

Question 19

Answer 19

Acid fast

Ziehle Neelsen Technique

breaks down mycolic acid(waxy lipid) - pink

nonacid fast as blue cocci

Question 20

Disinfectant

Answer 20

Used on objects

exampole Lysol and Bleach

Question 21

Antiseptic

Answer 21

Is a disinfectant that is nontoxic and can be used on skin and mucous membranes,

Like Isopropyl alcohol and Hydrogen peroxide

Question 22

How does Ultraviolet radiation control growth

Answer 22

Its a form of electromagnetic radiation 10-400nm that damages microorganisms DNA by creating thymine dimers which disort DNA.

Can be fixed by light and dark repair

Question 23

Coliforms

Answer 23

All aerobic and facultative anaerobic Gram negative rods which produve acid and gas from lactose fermentation in 48 hours at 35 C

Question 24

MPN

Answer 24

Most probable Numer

Question 25

Typical Coliform group #1

Answer 25

Forms dark purple colones with green metallic sheen

Question 26

Typical coliform group 2

Answer 26

2,3 butanediol fermenters with lower acid forming pink to purple colonies

Question 27

non coliforms

Answer 27

Clear colorless colonies

Question 28

Araga used for confirmed Test

Answer 28

Eosin Methylene Blue(EMB) a differential medium and selective medium

Question 29

Slime Mold and cytoplasmic streaming

Answer 29

Used Physarum Polycephalum in a tap water agar plates(TWA) wiith rolled oats Cytoplasmic streamein is the flow of oxygen and nutrents from one part of teh plasmoduim to another protoplasic vessel. Food

Question 30

Sterile Technique Steps

Answer 30

1) Light bunsen burner 2) Flame sterilze the loop inoculating 3) Pick up tube at a with an angle and hold cap then flame sterize teh lip of teh tube 4) hold steriliezed loop in the tube 5) pick a loopful of liquid 6 Flam sterilized teh lip of tube again and replace cap 7) Flame sterilzed teh loop again

Question 31

Motility procedure

Answer 31

Hanging Drop

Question 32

HyperTonic

Answer 32

More solutions outside the cell, water will flow to outside of cell and teh cell wtih dehydrate, skrink causeing plasmolysis.

Question 33

Faculatative halophiles or Halotorlerant

Answer 33

these organisms can live in normal conditions as well as moderately salty conditions up to 10% NaCl or hight an example is **Staphylococcus epidermidis**

Question 34

Obligate or extreem Halophiles

Answer 34

These micros MUST have very salty conditions at least 9% Nacl example is **Halobacterium salinarium**

Question 35

Hypotonic Solution

Answer 35

THere are more solutes inside teh cell and teh surrondign solutions has less water with flow into the cell to dilute the slutes this can cause bursting called **osmotic lysis**

Question 36

Isotonic

Answer 36

If the concentreation of solutes on teh outside of a cell equals the that of inside teh cell it si called isotonic solution (SAME)

Question 37

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Answer 37

Capsule Stain

Question 38

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Answer 38

Flagella Stain

Question 39

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Answer 39

Schaeffer Fulton Endospore stain

Question 40

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Answer 40

Cyrtal Violety Stain Endospore Stain

Question 41

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Answer 41

Fast Acide Ziechl Neelsen Stain