1
Q
how do we make e.coli cells competent
A
CaCl2 & temp shock = cell wall alterations = increase in permeability
2
Q
why do we want competent e.coli cells
A
plasmid uptake
3
Q
what are the plasmids used in practical 1?
A
pblt101 and pBluscript SK (+)
4
Q
what e.coli strain is used
A
JM105
5
Q
A
