Primary Hemostasis, Platelet Function. Lab Tests Flashcards Preview

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Flashcards in Primary Hemostasis, Platelet Function. Lab Tests Deck (74):
1

Cellular elements of hemostasis

Vascular intima
Extracellular tissue factor bearing cells
Platelets

2

Plasma components of hemostasis

Coagulation and fibrinolytic

3

Structure of blood vessel wall

Vascular initima

4

Rhomboid and contiguous

Endothelial cells

5

Anticoagulant properties of Vascular intima

Lamina propia
Nitric oxide
Heparin sulfate
TFPI
Thrombomodulin
PGI2 or prostacyclin
TPA

6

Activates fibrinolytic serum. Dissolves clot

TPA

7

Vasodilator, inhibits platelet activation

Prostacyclin

8

Activates protein C pathway. Digest factor Va and VIIIa

Thrombomodulin

9

Inactivates coagulation factor VIIa

TFPI

10

Inactivates extrinsic factor

TFPI

11

Retards coagulation by activating antithrombin

Heparin sulfate

12

Counteracts vasoconstriction

Nitric oxide

13

Relaxing factor

Nitric oxide

14

Promotes platelet activation and adhesion

Collagen

15

Origin of collagen

Fibroblast

16

Activates fibrin bound plasminogen to form plasmin

TPA

17

Digest thrombus and restore blood flow

Plasmin

18

Inhibits activation of fibrinolytic system

PAI-1

19

Adhesion molecules

P-selectin
ICAM
PECAM

20

Induce vasoconstriction

Smooth muscle cells

21

Who secretes vWF

Endothelial cells

22

Promotes leukocyte binding

ICAM
PECAM

23

Promotes platelets and leukocytes binding

P selectin

24

Platelets roll and cling to nonplatelet surfaces

Adhesion

25

Platelet adheres to each other

Aggregation

26

Platelets discharge the contents of their granules

Secretion

27

Absence of GP IIb/IIIa

Glanzmann thrombostemia

28

Absence of Gp Ib/IX

Bernard soulier syndrome

29

Where is vWF being stored

Weible palade bodies

30

Bind vWF

GP Ib/IX receptor

31

For jigsaw puzzle effect

Pseudopod

32

For crosslink to other platelet

GP IIb/IIIa

33

Requires fibrinogen

Aggregation

34

Requires extensive damage

Aggregation

35

Reversible aggregation
Platelets adhere loosely to each other

Primary aggregation

36

Irreversible aggregation
Release of substance that acts as antagonist

Secondary aggregation

37

Substances that promotes coagulation

HMWK
Fibrinogen
Factor V
Factor VIII:vWF

38

Substances that promotes aggregation

ADP
Calcium
PF4
Thrombospondin

39

Promotes vasoconstriction

Serotonin
Thromboxane A2

40

Promotes vascular repair

PDGF
B-thromboglobulin

41

Plasma precursors

Plasminogen

42

Plasma inhibitors

Alpha-2-antiplasmin

43

Complement system inhibitors

C1 esterase inhibitor

44

Positive result of capillary fragility test

Petechiae

45

Methods of Capillary fragility test

Tourniquet test
Rumple Leed
Gothlin
Quick

46

4+ petechiae

>50

47

1+ petechiae

0-10

48

2+ petechiae

11-20

49

3+ petechiae

21-50

50

Pinpoint hemorrhagic spots

Petechiae

51

Correlates with the degree of thrombocytopenia

Capillary fragility test

52

Original test for platelets

Bleeding time

53

Normal value of Duke method

2-4mins

54

Normal value of Ivy method

2-9mins

55

Factors affecting bleeding time

Vascular integrity
Platelet function
Platelet count

56

2 cuts in volar surface of hand

Ivy method

57

Reason why platelet count is difficult

Platelets:
.easily adhere to foreign surface
.Easily disintegrate
.difficult to distinguish from debris
.unequal distribution in circulation

58

Whole blood is diluted with diluting fluid in RBZc pipet and counted in hemocytometer

Direct platelet count

59

Platelets are counted in relation to 1000 RBC in the PBS

Indirect platelet count

60

Indirect methods

Damashek
Fonio
Olef

61

Direct methods

Guy and Leake
Reese Ecker
Brecker cronkite
Unopette

62

Direct using light microscope

Reese Ecker
Guy and Leake

63

Diluting fluid of Reese Ecker

Sodium citrate
BCB
Distilled water

64

Guy and Leake diluting fluid

Sodium citrate or oxalate
Crystal violet
Formalin

65

Diluent of brecker cronkite and use of diluent

1% ammonium oxalate
Hemolyzes RBC

66

Damashek diluent

BCB
Sodium citrate
Distilled water
Formalin
Sucrose

67

Best indirect method

Olef

68

Diluent of Fonio

14% MgSO4

69

Inhibits cyclopxygenase enzyme

Aspirin

70

Inhibit GP IIb/IIIa

Tirofibom

71

Inhibits ADP receptor

Clopidogrel bisulfate

72

Inhibit circulating thrombin

Heparin

73

Inhibit thrombin attach to fibrin

Hirudin

74

Process by which blood is maintained within the vessel walls

Hemostasis