Prokaryotic DNA replication

Question 1

In prokaryotic replication DNA is replicated…

Answer 1

prior to the mitosis phase of the cell cycle in the S phase:

equal DNA is passed to each daughter cell unlike viruses.

Question 2

How is DNA replicated?

Answer 2

Semi-conservatively meaning each strand is used as a template and each daughter DNA has an original strand.

Question 3

What are the 4 stages?

Answer 3

Initiation

Unwinding

Elongation

Termination

Question 4

What happens in initiation?

Answer 4

1) the process begins when an initiation protein know as DnaA bind to the locus oriC - a promoter region to being DNA transcription

2) Then a single stranded bonding protein Ssb hold the DNA bubble open

3) This is allows DnaB, which is a helicase, to use ATP to separate double stranded DNA

Question 5

What happens in Unwinding?

Answer 5

(After DNA helices unwinds the double strand and opens up template strand)

1) Supercoiling is prevented by DN gyrase - relaxes tension that increased during unwinding so DNA breakage doesn’t occur

Question 6

What happens in Elongation?

Question 7

What happens in Termination?

Question 8

What is the common organism prokaryotic replication is seen in?

Answer 8

Ecoli