proteins⭐️ Flashcards
explain how amino acids are linked together to form proteins
amino acids form dipeptides and then polypeptides via a condensation reaction, forming peptide bonds. the water is formed from the OH group of the carboxylic acid on one amino acid and the H in the amine group of anther amino acid. this sequence of amino acids is called a primary structure
explain how polypeptides are arranged to form a secondary structure of a protein
hydrogen bonds form between the OH group of a carboxylic acid and the H group on the amine group causing the polypeptide chain to twist into an alpha helix or fold into beta pleated sheets
explain how the secondary structure of a protein forms the tertiary structure of the protein
the specific 3D structure of a protein, formed by either hydrogen bonds, disulfide bridges and ionic bonds
explain how to quaternary structure of a protein is formed
the large protein molecule is formed from two or more polypeptide chains and additional prosthetic group (non protein)
describe a test for proteins
add sample to dilute sodium hydroxide. add very dilute copper sulphate and mix. a positive test is purple colour change
why might two proteins have different tertiary structures if they have the same number and type of amino acid
different primary structure/different sequence of amino acids. different disulfide bridges, ionic or hydrogen bonds formed
What bond forms between amino acids?
Peptide bonds
How are polypeptides formed?
Amino acids linked together via a condensation reaction, forming peptide bonds and releasing a molecule of water
How are disulfide bridges formed?
Two molecules of cysteine come close together so the sulfur atom on one cysteine bonds to the sulfur atom of the other.
What are the four functions of proteins?
Enzymes, antibodies, transport proteins, structural proteins
What is a structural protein?
They consist of long polypeptide chains lying parallel to each other with cross links between them. An example is keratin and collagen. They’re physically strong
Discuss transport proteins as a function of proteins?
Present in the cell membranes such as channel proteins containing hydrophobic and hydrophilic amino acids which cause the protein to fold up and form a channel. Allows ions across the membrane
Discuss antibodies as a function of proteins
Involved in the immune response. Made up of two short polypeptide chains and two long polypeptide chains bonded together. They have variable regions