Reproductive Flashcards
(16 cards)
What gas is used to inflate the abdomen for laprascopic procedures?
5% CO2 or 5% nitrogen
What are two methods to sample testis tissue?
Surgical wedge resection or biopsy with hooked 25 gauge needed.
Describe McGlinn’s technique of castration in GPs.
Removes seminiferous tubules and Leydig cells from tunica albuginea, leaving the epididymis and its nerve and vascular supply intact. Valuable for studies concerned with repro physiology of spermatozoa, the epididymis, and vas deferens.
How can spermatozoa be collected from male mice?
Traditionally via antemortem or postmortem procedure.
For serial samples, can flush uterine horns of euthanized post-coitus female or flush uterus via the vagina in an anesthetized female.
What occurs with rectal probe ejaculation in mice?
Secretion of vesicular and coagulating glands.
What finding on vaginal smear is indicative of ovulation in most species? What species is unique?
Most species have well-defined cornified epithelial cells. Rabbits cyclic changes in cytologic characteristics can be highly variable.
How many sperm should be inseminated?
At least 10^6 = 10,000,000 = 10 million
In what species has introduction of semen directly into the oviduct via laparoscopy been demonstrated?
Swine
What method of pregnancy detection is used primarily in primates? What is palpated?
Rectal bimanual palpation.
First palpable sign is dorsoventral rounding of anterior aspect of the body of the uterus.
What are potential complications of rectal palpation for preg determination?
Early embryonic abortions if performed prior to implantation or excess manipulation of the uterus occurs soon after implantation.
What is the earliest that pregnancy can be detected via rectal palpation?
30 days from onset of the last cycle. Recheck should be performed 2 weeks after initial positive palpation.
What type of uterus is associated with poor breeding performance in NHPs?
A long, 7mm wide ‘worm’ uterus
What is the current standard for evaluating pregnancy in NHPs?
Ultrasonic confirmation.
Radiographs used, but most useful following fetal calcification
What do chemical pregnancy tests for NHPs capture?
Urinary chorionic gonadotropin.
How early can pregnancy in the domestic cat be determined via palpation and elevated serum progesterone?
Palpation: 21-26 days gestation
Progesterone: 6 days after breeding
Why are sheep typically used as models of intrauterine fetal surgery?
Developing lamb is close in size to a human fetus and sheep usually only have 1-2 fetuses at a time.
