Required Practical 8 Flashcards
(23 cards)
Why does osmosis not occur in this case?
Osmosis does not occur because water potentials are the same.
What happens to the chloroplast if water potentials are the same?
Chloroplast does not burst / lyse / shrivel / shrink.
What would happen to the chloroplasts if water potentials were not the same?
Chloroplasts would burst / lyse / shrivel / shrink.
Why is it important to show that light does not affect DCPIP in the experiment?
To show that light does not affect DCPIP.
Why is it important to show that chloroplasts are required in this experiment?
To demonstrate that chloroplasts are essential for the reaction.
What happens to DCPIP in the presence of light and chlorophyll?
DCPIP is reduced by electrons from chlorophyll.
Where do the electrons that reduce DCPIP come from?
The electrons come from chlorophyll or the light-dependent reaction.
Why is a blender used to break open cells in this experiment?
A blender breaks the cell walls and releases chloroplasts.
What does the blender do to the chloroplasts and enzymes?
The blender produces friction and heat, but enzymes may be denatured, though chloroplasts remain functional.
Why should the solution be filtered or centrifuged?
To separate the chloroplasts from other cell components.
Why is centrifuging done at different speeds?
To isolate chloroplasts at a low speed and then separate them more effectively at a high speed.
What is collected after centrifuging?
The pellet (or sediment) containing the chloroplasts is collected.
Why is it important to keep the experiment in the dark?
To slow the release of electrons from chlorophyll and stop photosynthesis.
What would be observed if the color change in the experiment was due to isolation medium?
The color change would not occur in tube B, as it shows the effect of chloroplasts/chlorophyll.
How does ammonium hydroxide affect electron transport?
Ammonium hydroxide slows/stops electron transfer in the electron transport chain.
How does ammonium hydroxide affect photosynthesis?
It affects pH and can denature enzymes involved in the electron transport chain, stopping photosynthesis.
Why is it essential to compare different concentrations or chemicals in the experiment?
To identify the most effective weed killer or the most effective concentration.
What is the benefit of comparing the speed of decolorization?
It helps determine which chemical or concentration is most effective and saves time.
What effect does the weed killer have on the crop?
The weed killer is absorbed by the crop, reducing growth and yield.
How does the weed killer affect wildlife?
It has toxic effects on wildlife when the crop is consumed.
What happens to ATP production when there is no light or electron transfer?
Less or no ATP is produced.
What is the effect on reduced NADP production without light or electron transfer?
Less or no reduced NADP is produced.
What happens to the conversion of GP to TP without light?
Less or no GP is reduced or converted to TP.
