RNA Test Flashcards

(18 cards)

1
Q
A
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2
Q

Central Dogma

A

The flow of genetic information within a cell (DNA —> RNA —-> Protein)

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3
Q

RNA

A

-Ribonucleic acid

-Nucleic acid made of nucleotides

-Single stranded

-RNA nucleotide:

ribose sugar

phosphate group

nitrogenous base

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4
Q

mRNA

A

The type of RNA that carries copies of instructions for the assembly of amino acids into proteins from DNA to the rest of the cell

-Involved in both transcription and translation

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5
Q

mRNA’s role in transcription

A

serves as the primary RNA product carrying genetic information from DNA in the nucleus to the ribosomes in the cytoplasm, where it is used to synthesize proteins

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6
Q

mRNA’s role in translation

A

acts as a template, carrying the genetic code from DNA to ribosomes, where it is read to synthesize proteins

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7
Q

tRNA

A

The type of RNA that carries each amino acid to the ribosome during protein synthesis

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8
Q

tRNA’s role in translation

A

In translation, tRNA molecules act as adaptors, delivering the correct amino acids to the ribosome based on the mRNA codons.

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9
Q

rRNA

A

-Type of RNA that carries each amino acid to a ribosome during protein synthesis

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10
Q

rRNA’s involvement in transcription and translation

A

The ribosomes are composed of rRNA and these rRNAs molecules hold ribosomal proteins in place and carry out the chemical reactions that join amino acids

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11
Q

Transcription

A

-Process from making mRNA from a DNA template [gene]

-Occurs in the nucleus

-Initiation

 -DNA polymerase binds with the prometer
 
 -DNA polymerase separates the strands of DNA

-Elongation

   -RNA polymerase builds the mRNA following the basic pair rules

-Termination

   -RNA polymerase reaches a termination sequence

 -RNA polymerase falls off the DNA
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12
Q

mRNA editing

A

-mRNA splicing

-Introns are spliced out of the RNA (stays in the nucleus)

-Exons are spliced together making the final  mRNA molecule leave the nucleus

-Poly A tail (3’)

-Abunch of adenines are added to the 3’ side to protect it from being broken down

-Modified guanine cap (5’)

 -Guanines are added to one end of the mRNA to protect it from being broken down

-Final mRNA molecule goes from the nucleus to the cytoplasm

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13
Q

Translation

A

-Process of making protein/polypeptides from the mRNA

-Occrus on the ribosomes in the cytoplasm

-Initiation:

-MRNA, the ribosome, and the first tRNA carrying the methionine bind together

-Elongation

-After the ribosome slides down, tRNAs read the next codon and bring the next amino acids that match that codon

-Termination

 -Stop codon is reached and ribosome detaches and amino acids form into protein/polypeptide
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14
Q

What molecules are involved in protein synthesis? What are their function?

A

-DNA provides the instructions

-mRNA carries the genetic code

-tRNA delivers amino acids

-rRNA forms ribosomes (where protein synthesis mainly happens)

-Amino acids are the building blocks of proteins

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15
Q

Codon

A

-Location: mRNA

-a sequence of three consecutive nucleotides in mRNA that specifies a particular amino acid during protein synthesis.

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16
Q

Anticodon

A

-Location: tRNA

  • sequence of three nucleotides found on a transfer RNA (tRNA) molecule that recognizes and binds to a specific codon on a messenger RNA (mRNA) molecule during protein synthesis
17
Q

Point mutations

A

-Gene mutation in which a single base pair in DNA has been changed

18
Q

Frameshift mutation

A

Mutation that shifts the “reading frame” of the genetic message by inserting or deleting a nucleotide