S1 [LAB]: Tests for Glucose (Qualitative & Quantitative))

Question 1

Identify the test for glucose determination:

In hot alkaline solution, glucose reduces copper salts to copper oxide

Answer 1

Benedict’s test

Question 2

The Benedict’s reagent for glucose testing is made up of what component/s?

Answer 2

Copper sulfate
Sodium citrate
Sodium carbonate

Question 3

Fehling’s solution A for glucose testing is made up of what component/s?

Answer 3

Cupric sulfate

Question 4

Fehling’s solution B for glucose testing is made up of what component/s?

Answer 4

Rochelle’s salt
KOH

Question 5

Give the grading for the Benedict’s and Fehling’s Test for glucose:

No change in color

Answer 5

(-); Negative

Question 6

Give the grading for the Benedict’s and Fehling’s Test for glucose:

Green opacity and no precipitate

Answer 6

(+/-); Trace

Question 7

Give the grading for the Benedict’s and Fehling’s Test for glucose:

Green solution with yellow precipitate

Answer 7

(+); Positive

Question 8

Give the grading for the Benedict’s and Fehling’s Test for glucose:

Green to yellow solution with yellow precipitate

Answer 8

(++); Positive

Question 9

Give the grading for the Benedict’s and Fehling’s Test for glucose:

Muddy orange solution with yellow precipitate

Answer 9

(+++); Positive

Question 10

Give the grading for the Benedict’s and Fehling’s Test for glucose:

Orange to brick red precipitate

Answer 10

(++++); Positive

Question 11

The strong positive reaction of Benedict’s and Fehling’s test for glucose

Answer 11

Orange to brick red ppt.

Question 12

Identify the method of glucose determination:

In a hot alkaline solution, glucose reduces bismuth salts to metallic bismuth

Answer 12

Nylander’s Test

Question 13

Positive reaction for the presence of glucose in the Nylander’s test

Answer 13

Black color

Question 14

Positive reaction for the presence of trace glucose in the Nylander’s test

Answer 14

Brown

Question 15

In the Nylander’s test for glucose, if the solution turns black after cooling, the reaction is due to ___

Answer 15

substances other than sugar

Question 16

Identify the glucose determination test:

Caramelization of glucose by strong alkali with the aid of heat

Answer 16

Moore-Heller’s test

Question 17

Reagent used in the Moore-Heller’s test of glucose determination

Answer 17

10% KOH solution

Question 18

Give the expected color for the Moore-Heller’s test of glucose determination:

< or equal to 1% sugar

Answer 18

Canary yellow

Question 19

Give the expected color for the Moore-Heller’s test of glucose determination:

1-2% sugar

Answer 19

Wine yellow

Question 20

Give the expected color for the Moore-Heller’s test of glucose determination:

2-3% sugar

Answer 20

Cherry color

Question 21

Give the expected color for the Moore-Heller’s test of glucose determination:

3-4% sugar

Answer 21

Rum color

Question 22

Give the expected color for the Moore-Heller’s test of glucose determination:

> 4% sugar

Answer 22

Dark brown or black

Question 23

Identify the test for glucose determination:

When heated with concentrated hydrochloric acid, fructose forms oxymethylfurfurol, which gives a red color with resorcin

Answer 23

Seliwanoff’s Test/ Borchardt’s Test

Question 24

Seliwanoff’s reagent used for glucose determination is composed of ___

Answer 24

Resorcinol
HCl

Question 25

Positive result for Seliwannof's test of glucose determination

Answer 25

Bright red color

Question 26

Borchardt's reagent for glucose determination is composed of ___

Answer 26

25% HCl Resorcinol crystals

Question 27

Positive result for Borchardt's test for glucose determination

Answer 27

Yellow ethereal layer

Question 28

Rubner's reagent for glucose determination is composed of ___

Answer 28

Lead acetate powder Concentrated ammonium

Question 29

Positive result for Rubner's test of lactose determination

Answer 29

Brick red color

Question 30

Positive result for Bial Orcinol test of glucose determination

Answer 30

Green color

Question 31

Identify the sugar being measured: Benedict's Test

Answer 31

Glucose

Question 32

Identify the sugar being measured: Fehling' Test

Answer 32

Glucose

Question 33

Identify the sugar being measured: Nylander's Test

Answer 33

Glucose

Question 34

Identify the sugar being measured: Moore-Heller's Test

Answer 34

Glucose

Question 35

Identify the sugar being measured: Seliwanoff's Test

Answer 35

Fructose

Question 36

Identify the sugar being measured: Borchardt's Test

Answer 36

Fructose

Question 37

Identify the sugar being measured: Rubner's Test

Answer 37

Lactose

Question 38

Identify the sugar being measured: Bial Orcinol Method

Answer 38

Pentose

Question 39

Overflow type of glucosuria

Answer 39

Pre-renal

Question 40

Which reducing sugar is of diagnostic significance?

Answer 40

Galactose

Question 41

Preferred specimen for fructose determination

Answer 41

First morning urine

Question 42

Give the ratio of rgts. 1 & 2 in Fehling's test

Answer 42

1:1 (equal parts)

Question 43

Components of Nylander's reagent

Answer 43

Rochelle salt 10% NaOH or KOH Bismuth subnitrate