Spermatogonial Stem Cells

Question 1

What are spermatogonial stem cells?

Answer 1

Adult stem cells within the male reproductive system responsible for maintaining continuous spermatogenesis, the process of producing sperm

Question 2

What is hard about isolating SSC?

Answer 2

There is low numbers of these in the testes

Question 3

What is in the culture to help SSC’s grow?

Answer 3

GDNF

Question 4

Do SSC’s complete spermatogenesis after being transplanted into the testis of immunodeficient mice?

Answer 4

Yes

Question 5

Human spermatogonia

Answer 5

A dark to Apale

Question 6

Mouse spermatogonia?

Answer 6

Asingle - Apair - Aal - differentaited sperm

Question 7

Human foetal testis as a model for prepubertal germ cell development study

Answer 7

Testis were given chemotherapy and the testes cells decreases

Question 8

What was the cisplatin and the testes study?

Answer 8

Decreased germ cell numbers
Decreased long term germ cell reduction after xenograft
Decreased spermatogonia and SSC number in human prepubertal testes

Question 9

Describe the Rhesus monkey study by Hermann et al., 2012?

Answer 9

Made monkeys infertile through chemotherapy and transferred SSCs. They then injected any sperm made into oocytes via ICSI.

Question 10

Birth of a live offspring from transplantation of prepubertal monkey testes study?

Answer 10

Took cryopreserved testes tissue from a monkey and grafted it to the back or scrotal skin of castrated monkeys. These then produced sperm and graft derived sperm where used to make Grady the monkey.