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Flashcards in Staining Procesures Deck (14):
0

How do you make a smear?

1Label clean slide
2Flame loop and obtain 1 loop ful of water
3 flame loop cool flame top of test tube and obtain small amount of bacteria
4. Add bacteria to water
5. Reflame loop and make sure tube reflamed and lid put on
6. Air dry
7. Heat fix

1

What is morphology of E.Coli

Rod shaped

2

What is morphology of Bacillus megatherium

Long rod shape

3

What is morphology of mycobacterium?

Small rods

4

What is the morphology of staphylococcus?

Grape like

5

How do you make gram stain?

1. Smear
2. Crystal Violet--> 1 minute
3. Rinse with water
4. Iodine --> 1 min.
5. Decolonize with Ethyl alcohol
6. Rinse with water
7. Add safes in ---> 1 minute
8. Blot dry --> view on oil immersion

6

Gram positive is what color?

Purple

7

Gram negative is what color

Reddish color

8

How do you do an acid-fast stain?

1.smear
2. Add carbon fuchsin --> microwave 1 minute
3. Cool, decolonize with acid alcohol --> 15 seconds
4. Rinse with water
5. Add methylene blue--> 1 min
6. Rinse with water
7. Blot dry --> view on oil immersion lens

9

What color is acid fast positive?

Bright pink

10

What color is acid fast negative?

Blue

11

How do you make a spore stain?

1. smear
2. Add malachite green --> microwave for 1 minute
3. Cool and rinse with water
4. Add safaris fir 45 Sec
5. Rinse with water
6. Blot dry view on oil immersion

12

What color is spore positive?

Green and red
Green(indicate spores)
Red is cell wall

13

What color is sore stain negative?

Red