U2 LAB: ACCESSIONING Flashcards
(84 cards)
1
Q
under Anatomic Pathology Laboratory Receiving
A
- Receiving
- Logging
- Accessioning/Numbering
2
Q
under Receiving
A
- Request form
- Specimen
3
Q
Specimen must always be accompanied by?
A
request form
4
Q
This refers to documenting all steps, all information in request form, and all specimens.
A
Logging
5
Q
This is the process of giving an identifying marker to a specimen.
A
Accessioning / Numbering
6
Q
Accessioning starts in the?
A
receiving area
7
Q
Accessioning is based on?
A
the time it was received
8
Q
Request form must include:
A
- Health facility name
- Hospital number
- Name of patient*
- Date/time of request*
- Age/date of birth*
- Sex*
- Address
- Contact no.
- Clinical abstract
- Laboratory investigation/request
- Specimen
9
Q
Clinical abstract includes?
A
- medical history of patient
- chief complaint
- details of the patient’s case
10
Q
Specimen
Routine Histopath
A
Tissue/organ
11
Q
Specimen
Cytology
A
smears
12
Q
Specimen
Immunofluorescence
A
renal and skin biopsies
13
Q
Specimen
fixative for Immunofluorescence
A
saline or liquid nitrogen fixative
14
Q
Specimen
Immunofluorescence uses this to detect specific locations in the tissue
A
antigens
15
Q
T/F: Immunofluorescence and Immunostaining uses the same fixative.
A
True
16
Q
Specimen
Rush Frozen Section
A
- unfixed specimen
- specimens for Hirschsprung disease
17
Q
Specimen
fixative for Rush Frozen Section
A
liquified carbon dioxide or liquid nitrogen
18
Q
This is used for very high magnifications or ultramicroscopic details of specimen.
A
Electron Microscopy
19
Q
Specimen Rejection Criteria
A
- condition is not ideal for test
- fixative is not sufficient
- wrong container
- long transport time
- wrong or incomplete filled request
- unlabelled or mislabeled specimen
20
Q
This refers to decaying processes.
A
putrefication
21
Q
Process of Logging and Numbering
A
- Verify info on request form
- Verify Specimen Label
- Enter data in logbook/LIS
- Accession the specimen
22
Q
Importance of Accessioning
A
- avoid mixing/switching
- track down specimen/results
- prevents mismanagement
- process right specimen
- accurate and timely results
- proper management/treatment at right time
- record keeping
- laboratory planning
23
Q
First step in histopathologic techniques
A
Accessioning / Numbering
24
Q
Purpose of Accessioning
A
identify specimen without writing the name of the patient
25
initial assessment of specimen
Accessioning
26
This is to provide criteria for reception, registration and handling of surgical pathological specimens.
Specimen reception, accessioning and handling
27
All specimens must be delivered to the laboratory within?
1 hour maximum
28
10% neutral buffer takes how many hours for complete fixation?
12 to 24 hours
29
If specimen is within the 1 hour range, put in?
automatic tissue processor (to ensure complete fixation)
30
Patient information in request form
Refers to where the specimen was collected
Type of specimen (anatomic site)
31
T/F: Location of patient must be seen in request form.
True
32
Clinical history of patient must include the following:
- laboratory results
- pertinent lab works
- previous biopsies
- treatment done
- gynecologic and menstrual history
33
This is used for pathologic diagnosis.
Biopsy
34
T/F: Surgery residents may sign the request form.
True
35
For send out forms to referral laboratories, what must be included?
- Name
- Address of physician
36
Specimen container must be labeled with the following:
- Full patient's name
- Medical record number
- Age and sex of patient
- Type of specimen (Anatomic site)
- Date and time of collection
- Location of patient (ward, patient ID/bed)
37
If birthday is not written, you may write?
medical record number
38
Where should the label in specimen containers be placed?
body of container
39
T/F: The one who collects the specimen will be the one to label the container.
True
40
Accession Number
S
Surgical specimen
41
Accession Number
A
Anatomic pathology
42
Accession Number
H
Histopathology
43
Accession Number
C
Cytology
44
The identity of every specimen is maintained at all time with two identifiers:
- MRN
- Pathology number
45
Used in identifying the case
Case accession number
46
Used if there are multiple specimens for the same patient case
Case specimen number (A,B,C)
47
Used if there are multiple blocks on the same specimen
Specimen block number (A1, A2, A3)
48
Used if there are multiple slides cut from a single block
Block slide number (1,2,3)
49
Refers to specimens that are cut before the physician is asking for a review
Recuts
50
All slides other than those stained by H&E will?
bear the name of the stain
51
Maintains records of blocks, slides, stains prepared, and is capable of demonstrating volumes for any given time
Quadramed
52
This refers to bacterial contamination or bacteria destroying the specimen cells
Putrefication
53
Cell is destroyed because of natural process of decomposition or enzymes inside the cell
Autolysis
54
This is common in specimens for slide reviews.
Damaged specimen or broken slides
55
T/F: The attending physician must be the one to enter the request on the computer.
True
56
All specimens from outside the hospital except government must have?
approval of Hospital administration and consultant
57
Requests for frozen section require ?
24 hours notice ahead of operation (result must be deployed before operation is finished)
58
Accessioning is carried out where and by who?
laboratory reception area, technologists
59
Specimen should be immersed in what fixative within 1 hour of biopsy?
10% buffered formalin
60
Volume of formalin should be at least _________ volume of the specimen.
10 times
61
All specimens must be fixed in 10% formalin except?
- Frozen section
- Immunofluorescence
- Electron microscopy
62
unfixed, fresh state
Frozen section
63
saline or liquid nitrogen
Immunofluorescence
64
2% glutaraldehyde solution in the cold after excision
Electron Microscopy
65
Renal biopsies
Before the procedure
call 15 minutes, immerse sample in saline
66
Renal biopsies
Examined under the dissecting microscope for?
glomeruli
- immunofluorescence
- glutaraldehyde for EM study
- formalin for paraffin processing
67
fixative for Testicular biopsies
Bouin's fluid
68
fixative for Electron microscopy
4% glutaraldehyde solution then refrigerate
69
surgical margins should be oriented by sutures
Breast Lump
70
The apical nodes should be indicated by sutures.
Mastectomy
71
In case of simple mastectomy, this is to be indicated by a separate suture
superior margin
72
These are closest lymph nodes to the origin of cancer / malignancy (undergoes metastasis)
Sentinel lymph node
73
Requisition for sentinel lymph nodes includes:
- procedure type
- date and time of injection
- location of lymph node
- tracers used
- radioactivity count
74
If possible, this should be included to ensure integrity of lymph node capsules and assess tumor spread.
Perinodal fat
75
Known infective specimen should be clearly marked with?
Red marker
76
The proximal or distal excised margins should be oriented by suture.
Bowel Resection
77
Received frozen and stored in deep freezer for immunoflurescence studies
Skin Biopsies
78
Normally submitted for processing on same day
Tiny and small biopsies (Endoscopy and Dermatology)
79
Specimens are usually kept at room temp for how many weeks after the official report is released?
2 weeks
80
Referral slides must be accompanies by the official report with following info:
- Patient's name
- Medical record number
- Hospital / clinic pathology number
- Number of slides / blocks
81
Specimens exempt from pathology examination
- foreign bodies
- hair, finger/toe nails (cosmetic reason)
- teeth, dental appliances
- lens
- calculi
- nasal septum cartilage, bone
- foreskin from circumcision (10y.o. and younger)
82
Specimens accepted for Gross Description only
- torn meniscus
- varicose veins
- bone fragments (non-pathologic fracture)
- ear cartilage
- fetus
83
This should be performed whenever there is a request by attending physician or at the discretion of the pathologist.
Microscopic examination
84
T/F: Specimens for Gross Descriptions are embedded in cassettes.
False
