W2/L2: Intro to histology & blood Flashcards
(24 cards)
What charge does haematoxylin bind to?
Negatively charged (aka basophilic)
What sort of structures does haematoxylin stain?
The phosphate groups of DNA/RNA
What colour is haematoxylin?
Blue/purple
What charge does Eosin bind to?
Positively charged
What sort of structures does eosin stain?
Amino acids
What does amphophilic mean?
The cell binds both types of dyes
Approximately how much blood would a 70kg person have?
5L
What are the components of plasma?
Water Protein Salts Lipids Sugar
What are the 3 main groups of proteins in plasma?
Coagulatory
Albumin
Globulins
What are erythrocytes
RBCs
About how many RBCs do we have per litre?
5 x 10^12 / L
What are the two groups of WBCs?
Granulocytes
Mononuclear leukocytes
Which cells are granulocytes?
Neutrophils
Eosinophils
Basophils
Which cells are mononuclear leukocytes?
Lymphocytes
Monocytes
Order the WBCs in descending concentration
Neutrophils Lymphocytes Monocytes Eosinophils Basophils
What is the haematocrit?
The proportion of blood volume that is RBCs
What is normal haematocrit?
45%
What are the key features of the RBC?
Biconcave disc
No nucleus or organelles
What’s the life span of RBCs?
~120 days
What’s a reticulocyte?
The immature form of RBCs first released into the blood
What proportion of the circulating RBCs are reticulocytes?
About 1%
What sort of things would cause an increased proportion of reticulocytes?
Haemorrhage or haemolysis
What’s a normoblast?
The immature, nucleated RBC
What are the characteristics of reticulocytes?
No nucleus
Some organelles
