Week 10- Part 7+8- Endurance Flashcards
(24 cards)
What is the approach to investigate the potential of a specific cell?
Genomics
What is the approach to investigate mRNA response to drug or exercise treatment?
Transcriptomics
What is the approach to measure different types of lipids?
Lipidomics
What is the approach to investigate post-translational modifications of proteins?
Proteomics
What is the protocol of mass spectrometry?
-Homogenise a bio sample to isolate the proteins
-unravel the proteins and chop them into peptides
-Peptides are converted from liquid to gas
-When it combines to ions we get information related to mass and amino acid abundance
Where are most the changes due to exercise observed?
at the mRNA level
In response to intense exercise, how many proteins are increased in phosphorylation?
1322
How many kinases does exercise stimulate in the skeletal muscle?
As many as 15
What are lifestyle diseases often associated with?
-increased visceral adiposity as a result of lack of movement and exercise
What does exercise training improve that protects against high fat diet induced hepatosteatosis?
-increase time to exhaustion
-increased muscle oxidative capacity
-glucose tolerance
What regulates fatty acid progression into the liver?
CD36 and FATP4 (channels)
What occurs to cytokines (TNF-R, IL-1ra, il-10) following a marathon?
Increase in a similar way to sepsis
In response to what do muscle cells release IL-6?
Contraction
What happens to IL-6 if you remove glycogen in the leg and exercise? What does it confirm?
Significant increase
-confirming that IL-6 is a myokine
What does IL-6 stimulate the release of?
Glucose from the liver
What is musclin?
A myokine that enhances endurance in mice
How does musclin enhance endurance?
Via PGC-1a mediated mitochondrial biogenesis
What is secreted when PGC-1a is overexpressed?
BAIBA
What happens when PGC-1a is overexpressed during endurance exercise?
-increase in UCP1
-More mitochondria in fat cells
What protein is released in circulation with exercise (exercise responsive)?
Cathepsin B
What happens when Cathepsin B is removed?
mental impairment
What is AAV9?
A virus to deliver edited gene constructs that don’t impact welfare (don’t give the mice a disease)
What does Turbo ID construct allow for?
cells to tag proteins that are secreted with biotin which is delivered in the diet in the weeks before training
