Week 5 - Content

Question 1

What is the Bradford Assay?

Answer 1

Uses chemical dye (Coomassie Blue) which interacts with proteins and changes colour dependant on the concentration of proteins in the sample.

Question 2

What is the dye used in the Bradford assay?

Answer 2

Coomassie Blue

Question 3

What technique is used alongside the Bradford Assay to determine the concentration of proteins?

Answer 3

Spectroscopy

Question 4

What does the blue colour in the Bradford Assay show

Answer 4

How concentrated the proteins are, the bluer the more concentrated.

Question 5

What are randomised controlled trials?

Answer 5

Where participants are randomly assigned to different groups to test the effect of an intervention, aiming to establish cause and effect relationship.

Question 6

What is a double blind randomised control trial?

Answer 6

Both the researcher and participants are unaware of what groups they have been placed in, aim is to prevent placebo effect.

Question 7

What is the placebo effect?

Answer 7

Improvement in patients condition after receiving sham or fake treatment.

Question 8

What is a placebo?

Answer 8

A sham or fake form of what is being offered.