11.06.13 Molecular Biology Transcription Flashcards Preview

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Flashcards in 11.06.13 Molecular Biology Transcription Deck (38):
1

What are the two difference between DNA and RNA?

RNA has ribose
DNA has deoxyribose

RNA has Uracil
DNA has thymine

2

RNA is mostly (double/single) stranded

single

3

hepatits delta virus come from (DNA/RNA)

RNA

4

T/F RNA as a genome

T

5

T/F RNA is carrier of information

T

6

T/F RNA deals with protein synthesis

T

7

T/F RNA has a catalytic funciton

T

8

(rRNA/tRNA/mRNA) most abundant

rRNA

9

(rRNA/tRNA/mRNA) less abundant

tRNA

10

(rRNA/tRNA/mRNA) least abundant

mRNA

11

RNA polymerase binds to (active site/promotor region)

promoter region

12

no primer is required
-no proofreadin occurs
--in (DNA/RNA) trancription

RNA

13

T/F in RNA transcritpion terminates at a hairpin loop

T

14

(e.coli/yeast/simplest polymerase) has a single polypeptide T3 and T7 bacteriophages

simplest polymerase

15

(e.coli/yeast/simplest polymerase) 5 subunits

e.coli

16

(e.coli/yeast/simplest polymerase) 12 subunits

yeast

17

(yeast/E Coli) RNA polymerase has a w subunit

e.coli

18

promotore stength = ____ of transcription

rate

19

T/F sequences of strong promotors are closer to conseus sequence

T

20

(e coli/yeast) 35 sequece , 10 sequence promotor . Start point is oncidered +1

e. coli

21

(core enzyme/holoenzyme) can bind any DNA

core enzyme

22

(core enzyme/holoenzyme) forms and binds a promoter closed complex

holoenzyme

23

(holoenzyme/polymerase) unwinds the DNA open complex

polymerase

24

what is the connection between drug resistance, antibiotics and trascription??(helicase/topoisomerase) inhibitors

topoisomerase

25

what is the connection between drug resistance, antibiotics and trascription??(helicase/topoisomerase) inhibitors

topoisomerase

26

(Novobiocin/Ciprofloxacin)
Quinolone antibiotic Binds to the A subunit of bacterial DNA gyrase.
DNA cleavage and resealing activity impaired.
No sealing of double-strand breaks: DNA damage.

ciprofloxacin

27

(Novobiocin/Ciprofloxacin)

Coumarin antibiotic
inhibits bacterial DNA gyrase by binding to the ATP binding site in its B subunit.
Inhibits its DNA supercoiling activity.
Transcription of many bacterial genes disrupted

novobiocin

28

what are some differences in prokaryotic vs eukaryotic transctiption?

structure of RNA polymerase
promoter sequence
bacteria have operon
eukaryotic genes are individually regulated

29

(eukaryotes/prokaryotes) RNa polymerase does not bind promoter directly

eukaryotes

30

(eukaryotes/prokaryotes) RNa polymerase does not bind promoter directly

eukaryotes

31

(eukaryotes/prokaryotes) RNa polymerase does bind promoter directly

prokaryotes

32

(rifampin/Alpha-amanitin) inhibits e.coli

rifampin

33

(rifampin/Alpha-amanitin) inhibits human mitochondrial

riampin

34

(rifampin/Alpha-amanitin) inhibits RNA pol 1,ii,iii

alpha amanitin

35

(rifampin/alpha-amanitin) Drug blocks the initiation of transcription by binding to a protein pocket near the active site of the β subunit of the bacterial polymerase to block the path of the nascent RNA during initiation

rifampin

36

what are tehe two important parts in a actinomycin D transcription inhibitor

oligopeptide tides, phenoxazone ring

37

actinomycin d block movement of (DNA/RNA) polymerase

RNA

38

phenoxazone ring gts intercalagted between two (CG/AT) pairs

CG