11-4-13 DNA replication Flashcards Preview

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Flashcards in 11-4-13 DNA replication Deck (20):
1

(Southern/Northern) hybridization of a probe fragment to RNA fragment

norther

2

(southern/northern)hybridization of a probe fragment to a DNA target

southern

3

T/F PCR quantifies DNA

T

4

when two fragment of DNA base pair with each other its called

hybridization

5

A (PCR/microarray-chip) allows us to see if there is a difference in gene expressin pattern between responders and non-responders of drugs

microarray chip

6

T/F numbers of repeats in the VNTR is highly polymorphic in the population

T

7

the (PCR/VNTR) repeats units that are short intermediate or large

VNTRs

8

what does VNTR stand for ?

variable number of tandem repeats

9

T/F every cell in teh body arises form an existing cell

T

10

Basic characteristic such as DNA replication i suniversal to al cells

T

11

T/F Dna replication only when conditions are right

T

12

Mitosis occurs only after replication has completed

T

13

T/F cytokinesis occurs only after all chromosmes have separated

T

14

DNA replication is (conservative/semiconservative)

semiconservative

15

DNA replicates (3'->5'/5"->3')

5'->3'

16

T/F e. coli DNA polymerase carries out DNA replicaiotn

T

17

DNA polymerase (1/2) low processivity dna repair

DNA polymerase 1

18

DNA polymerase (1/2) somewhat high processivity DNA repair

DNa polymerase 2

19

T/F primers are absolute requirement for replicaiton

T

20

(topoisomerase/helicase) relieve stress on DNA by making transiet cuts on DNA and chage link number

topoisomerase