11-4-13 Replication and Telomeres Flashcards Preview

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Flashcards in 11-4-13 Replication and Telomeres Deck (50):
1

T/F mammalian and E. coli replicaiotn processes are similar

T

2

T/F all component of repliaciont and mutations in genes responsible for replication have been characterized in yeast

T

3

-similar replication fork geometry
-mulitprotein replication machine
are the same in (yeast/e coli./mammalian)

e.coli and mammalian

4

Genome- 4.64 million basepairs circular DNA
Single origin of replication
Same polymerase for leading and lagging strand synthesis
describes (e.coli/mammalian)

e. coli

5

23 pairs of linear chromosomes- 3.2 billion basepairs
Multiple origins of replication (~1000)
Same or different polymerases for leading and lagging strand synthesis
describes (e.coli/mammalian)

mammalian

6

single origin of replication
-describes (ecoli/mammalian)

e.coli

7

same polymerase for leadig and lagging strand
-describes (ecoli/mammalian)

e.coli

8

multiple origins of replication
-describes (ecoli/mammalian)

mammalian

9

same or different polymerases for leading and lagging strand synthesis
(e.coli/mammalian)

mammalian

10

RNaseH degrades (DNA/RNA)

RNA

11

T/F okasaki fragments are smaller than E. coli

T

12

polymerase (alpha/beta/gamma) is primase

alpha

13

polymerase (alpha/beta/gamma) is mitochondrial replication

gamma

14

polymerase (alpha/beta/gamma) is repair

beta

15

polymerase sigma and e are both ____

nuclear replication

16

(e.coli/mammalian) replication is cirlcular

e.coli

17

(e.coli/mammalian) replication is linear

mammalian

18

which end will shorten with each replication cycle?
5' or 3'

5'

19

-coding sequences may be eventualy lost
-senescence or death signal may be triggered
--these are both consequence of what?

shortening of the 5' end after each replication cycle/

20

-origin of repliication
-centromere
-telomere
--these are all requirement for what

chromosome replication

21

(centromere/teleomore) seal the ends of chromosomes

telomere

22

-avoid undesirable fusion
-aboid aberrant recomination
---(centromere/teleomere)

telomere

23

(centromere/teleomore) attach chromosomes to nuclear envelope

telomere

24

telomeres have (sepa/hexa)meric repeats

hexameric

25

___ makes teomeric repeats

telomrase

26

what is a telomerase?

protein + RNA

27

what type of activity does the protein portion of the temoerase do?

reverse transcriptase

28

telomerase is (DNA/RNA) dependent DNA polymerase

RNA

29

which end does telomerase extend?

3'

30

telomere repeats from G (quartets/triplets)

quartets

31

what is the name of the tail made by the telomerase (A tail/G tail)

G-tail

32

(somatic/germ) cells have detectable activity

germ

33

(somatic/germ) cells have no detecable activiyt

somatic

34

fibroblast from the (young/elderly) have shorter fibroblasts

elderly

35

-replicative sensecense
-apoptosis
--are induced by (long/short) telomeres

short

36

T/F in human cnacer there is more telomerase activity than in normal tissue

T

37

GRN163L and G-quadruplex stabilizing agens are thoguh to (activate/block) the replicative portention of cancer cells

block

38

Viruses (have/don't have) a cell structure or metabolism, but they (are/aren't) capable of reproducing viruses

dont have, aren't

39

____ is an inert virus particle outside of teh cell host

virion

40

virus genomes are (single/double) DNA and RNA

single double

41

T/F viral nucleic acids are replicated only in host cells using host cell machinery

T

42

Replication of viruses involves : what three things?

synthesis of genome, translation , transcription

43

Viral genomes (are/aren't) variable in size organization and replication patterns

are

44

HIV has
A) 9749 nucleotides 9 genes 15 proteins
B) 9749 nucleotides 15 genes 9 proteins

A

45

AZT inhibits (reverse transcriptiase/integrase)

reverse transcriptase

46

bacterial gyrase inhibitors are used in (antibiotics/chemotherapy)

antibiotics

47

topoisomerase inhibitors are used for (antibiotics/chemotherapy)

chemotherapy

48

5-Florouracil inhibitor of (DNA replication/reverse transriptase) used for cancer chemotherapy

DNA replication

49

5-florouriacil is an analogue for uracil and ___

thymine

50

Capecitabine is a orally administered chemotherapeutic agent which is converted to (5-florouracil/AZT)

5-Florouracil