Chapter 8 - Microscopy and Lab Techniques

Question 1

Fixation

Answer 1

process of getting cells to stick on a microscope slide

Question 2

Heat fixation

Answer 2

Cell placed on one side of microscope slide, heat applied with Bunsen burner, cells are stained

Question 3

Optical microscopy

Answer 3

shine light on a sample, light reflects and pass through series of lenses which magnify the object; can be used to observe living cells

Question 4

Electron microscopy

Answer 4

bombard sample with electrons, electrons bounce off the sample and pass through series of magnetic fields and land on a screen. Viewed on a computer (Indirectly viewing)

Question 5

Electron microscopy requires cells to be:

Answer 5

fixed, stained, and killed before being observed

Question 6

Which offers higher resolution - electron or optical microscopy?

Answer 6

Electron microscopy

Question 7

Stereo microscope

Answer 7

low magnification to observe the surface of live specimens

Question 8

Compound microscope

Answer 8

more than one lens; can be used to view simple, one-cell thick live cells

Question 9

Drawback of compound microscopy

Answer 9

poor contrast - however, fixing/staining the cell would kill it

Question 10

Bright field microscopes

Answer 10

compound microscopes that have a bright light to illuminate the sample

Question 11

Phase contrast microscopes

Answer 11

allow the visualization of thin samples containing live cells; have tremendous contrast abilities