ELISA

Question 1

Indirect ELISA?

Answer 1

Antigen coated well, add specific antibody then add enzyme conjugated secondary antibody and add substrate and measure colour

Colour is proportional to the amount of a specific antibody

Question 2

Sandwich Elisa?

Answer 2

Antibody coated well then add antigen to be measures, add enzyme conjugated secondary antibody then add substrate and measure colour

Colour proportional to the amount of antigen e.g cytokine, tumour marker

Question 3

How does sandwich work?

Answer 3

AntiIgG then add plasma containing IgG then different anti igG binding on a different site

Question 4

Pregnancy tests for?

Answer 4

Antigen- human chorionic gonadotrophin

Question 5

Why do you block with milk?

Answer 5

So there is specific binding of the antibody and the antibody doesn’t bind to the plastic

Question 6

The typical shapes of a calibration curve is?

Answer 6

Sigmoid, because it might not be sensitive enough or there’s too much antibody