Anitgen P&P- Pathways

Question 1

What are the following molecules important for?
Invariant chain
HLA-DM
HLA-DO
MARCH-1

Answer 1

MHC class 2 antigen processing and presentation

Question 2

What fuses with the phagosome to form the MHC class 2 molecule?

Answer 2

Lysosome

Question 3

What fuses with the lysosome/phagosome fused molecule to form the MHC class 2 molecule?

Answer 3

Endosome with CLIP

Question 4

What catalyzes the release of CLIP and the binding of antigenic peptides in MHC 2 compartment (MIC)?

Answer 4

HLA-DM

Question 5

HLA-DO acts as a NEGATIVE regulator of ___-__

Answer 5

HLA-DM

Question 6

DR binds a peptide —> DM allows peptide exchange —> DO ________ DM from editing MHC 2 molecules

Answer 6

Prevents

Question 7

What regulates MHC class 2 antigen processing and presentation?

Answer 7

MARCH-1

Question 8

Which MHC class are the following molecules important for?
-Transporters associated with antigen processing (TAP)
-Endoplasm9c reticulum amino-peptidase associated with antigen processing (ERAAP)
-Proteosome

Answer 8

1

Question 9

Which MHC class 1 molecule is responsible for transporting peptides generated from proteasome, that are from the cytosol, to the ER?

Answer 9

TAP

Question 10

What do TAP 1 and 2 form?

Answer 10

Heterodimer

Question 11

What is the expression of TAP enhanced by?

Answer 11

Interferon

Question 12

The amino termini of the peptides produced by proteasome are too long and need to be trimmed by ______ before binding to MHC 1 molecules?

Answer 12

ERAAP

Question 13

Whose expression is increased by IFN-y?

Answer 13

ERAAP

Question 14

What has a 20S catalytic core and 2 19S regulatory caps?

Answer 14

Constitutive proteasome

Question 15

What are the 2 outer ring subunits called located on the 20S catalytic core of the constitutive proteasome?

Answer 15

Alpha (7 of them)

Question 16

Are the two outer rings- Alpha units- catalytic or not catalytic?

Answer 16

Not catalytic

Question 17

What are the 2 inner ring subunits called located on the 20S catalytic core of the constitutive proteasome?

Answer 17

Beta (7 of them)

Question 18

For Ubiquitin-proteasome system (UPS)-
What needs to be added with ubiquitin before processed by proteasome?

Answer 18

Proteins

Question 19

What chain of UPS is recognized by the 19S cap?

Answer 19

Ubiquitin chain

Question 20

What proteasome is induced by interferons and
PA28 proteasome-activator complex is induced by
IFN-y?

Answer 20

Immunoproteasome

Question 21

What type of subunits are regulated accommodate antigen processing needs?

Answer 21

Proteasome

Question 22

Exogenous antigens may enter the cytoplasm joining the ____________ antigen pathway, and vice versa

Answer 22

Endogenous

Question 23

What 2 pathways interact extensively in the antigen processing pathways?

Answer 23

MHC 1 and 2

Question 24

What type of antigens can be loaded on to MHC 1 molecules- CROSS PRESENTATION?

Answer 24

Exogenous

Question 25

Only _________ DC subset can efficiently perform cross-presentation

Answer 25

Specific

Question 26

What type of antigens are loaded on MHC 2 molecules- AUTOPHAGY PATHWAY

Answer 26

Endogenous

Question 27

Which pathway has antigens that are processed into small peptides by the proteasome in the cytoplasm?

Answer 27

MHC 1

Question 28

What pathways is: ingested proteins are broken up by lysosomal proteases into peptide fragment of varying lengths?

Answer 28

MHC 2