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MTAP 1 Bacte > (Part 2) 2.2.3 Strep Media and Test > Flashcards

(Part 2) 2.2.3 Strep Media and Test Flashcards

1
Q

Which of the following cant grow Strep spp.?

SBA
CHOC
PEA
CNA

A

None

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2
Q

Abiotrophia and Granulicatella cannot grow in SBA

T or F

A

T

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3
Q

Abiotrophia and Granulicatella requires what vitamin to be grown?

A

Vitamin B6

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4
Q

Used for Isolating GAS from Throat Swabs? (Full name)

A

5% Sheep blood Agar
w/
Trimetophrim-Sulfamethoxazole (SXT)

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5
Q

LIM broth isolates what strep?

A

Group B strep

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6
Q

LIM broth is a Todd-Hewitt broth that contains?

A

CNA (Colistin Nalidixic acid)

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7
Q

A selective - Differential based on esculing hydrolysis that isolates enterococci?

A

Enterococcosel agar

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8
Q

What is the Sus ZOI for Taxo P?

What is Taxo P?

A

> 14mm

Optochin

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9
Q

What is the Sus ZOI for Taxo A

What is Taxo A?

A

> 10mm

Bacitracin

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10
Q

What is the reagent of PYR?

A

Pyrolidonyl-Naphthylamide

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11
Q

What is the positive indicator for PYR?

A

Bright Red

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12
Q

What is the meaning of CAMP?

A

Christie-Atkins-Munch-Peterson

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13
Q

What is the Streaked organism (Control) used for CAMP [Vertical]?

A

S. aurues

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14
Q

What is the result for CAMP?

A

Arrowhead

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15
Q

CAMP is used to determine what strep?

A

S. agalactiae

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16
Q

S. aureus act as a source of ______ in CAMP

A

Beta lysin

Enhance Beta lysin for Group B

17
Q

What media is used for CAMP?

A

BAP

18
Q

What is the reagent for Hippurate hydolysis

A

Hippuric acid / Benzoic acid

19
Q

What happens to hippuric acid when is being hydrolysed

A

Hippric acid -> glycine

20
Q

Positive indicator for Hippurate hydrolysis

A

Purple

21
Q

What strep is positive for Hippurate hydrolysis?

A

Strep agalactiae

22
Q

What is the inhibitor used in Bile esculing hydrolysis?

A

40% bile

23
Q

In bile esculin, what is used to differentiate Enterococci to other strep?

A

Esculin

24
Q

What is the positive indicator for Bile esculin?

A

Blackening of agar

25
Q

What is the reagent for Salt tolerance test?

A

6.5% NaCl broth

26
Q

What is the positive indicator for Salt tolerance test?

A

Turbidity

27
Q

What is the reagent used for Bile solubility test?

A

Na deoxycholate and Na taurocholate

28
Q

What is the positive indicator for Bile solubility test?

A

Lysis of colonies

29
Q

What is the reagent for Optochin test

A

Ehtylhydrocuprene hydrochloride

30
Q

Size and grams of Ethylhydrocuprene hydrochloride?

A

6mm, 5ug disk

31
Q

What bacteria is positive in Optochin?

A

S. pneumoniae

32
Q

What bacteria is positive in Bile solubility?

A

S. pneumoniae

33
Q

This has a positive result for Capsular swelling?

Antiserum for capsule

A

Neufeld reaction (Quellung reaction)

34
Q

S. pneumoniae has capsule

T or F

A

T

MTAP 1 Bacte (93 decks)

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