(Part 2) 2.9.3 Nocardia microscopy and media

Question 1

Nocardia is Gram?

Answer 1

Pos

Question 2

What is the characteristics of Nocardia in microscope?

Answer 2

Beaded branching filaments

Remember na similar siya sa fungi

Question 3

Since Nocardia is Partially acid fast. What is the concentration of the decolorizer?

Answer 3

0.5% to 1% sulfuric acid

(Reference: 3% sulfuric acid and 95% Ethanol)

Question 4

Where can granules be seen in what type of infection?

Answer 4

Cutaneous infection

Question 5

Sabouraud dextrose agar is usually used for fungi. Can this help grow Nocardia?

Answer 5

Yes

Question 6

Tween 80, SBA and Lowenstein-Jensen are agar can be used for Nocardia

T or F

Answer 6

F

Tween 80 is not used

Question 7

BHI is a broth that is used for Nocardia

T or F

Answer 7

T

Question 8

Like Majority of pathgenic bacteria, Nocardia can grow at 35C, What is the temp to increase recovery of Nocardia?

Answer 8

30C

This temp is similar to help grow Fungi

Question 9

Selective and nonselective agars should be incubated at 35C and 30C, this means how many agar plates should be used?

Answer 9

4

1 select and 1 nonselect on each temp

Question 10

Duration of Nocardia plates to be incubated?

Answer 10

2-3 weeks

Question 11

What is the colony characteristics for Nocardia?

Answer 11

Chalky, Matte , Velvety appearance

(Breadcrumbs appearance)