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MTAP 1 Bacte > (Part 2) 2.22.1 Anaerobes media and incubations > Flashcards

(Part 2) 2.22.1 Anaerobes media and incubations Flashcards

1
Q

Anaerobes lacks superoxide dismutase and catalase which are enzymes required to breakdown nitrogen

T or F

A

F

Not N its oxygen

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2
Q

Prevotella

O. anaerobes / Aerotolerant anaerobes

A

O anaerobes

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3
Q

Actinomyces

O. anaerobes / Aerotolerant anaerobes

A

Aerotolerant

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4
Q

Bifidoacterium

O. anaerobes / Aerotolerant anaerobes

A

Aero

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5
Q

Fusobacterium

O. anaerobes / Aerotolerant anaerobes

A

Obligate

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6
Q

Bacteroides

O. anaerobes / Aerotolerant anaerobes

A

Obligaate

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7
Q

Clostridium

O. anaerobes / Aerotolerant anaerobes

A

Aero

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8
Q

Best specimen for anaerobes are Tissue biopsy and Needle Aspiration

T or F

A

T

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9
Q

Swabs are recommended for Anaerobes sample collection

T or F

A

F

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10
Q

Anaerobic media contains

Hemin
Blood
Vitamin E

T or F

A

F

Vitamine K

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11
Q

Anaerobic media that provides a source of CO2

A

Sodium Bicarbonate

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12
Q

Purpose of reducing agents?

A

Absorb oxygen

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13
Q

is used for isolation of all anaerobic organism including obligate and facultative anaerobes.

  1. Anaerobic blood agar
  2. Anaerobic PEA blood agar
  3. Anaerobic kanamycin-vancomycin blood agar
  4. Anaerobic paromomycin-vancomycin laked blood agar (PVLB)
  5. Thioglycollate
  6. Bacteroides bile-esculin (BBE)
A

1

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14
Q

The antibiotic
vancomycin will inhibit facultative and obligate gram positive anaerobes while kanamycin inhibits the growth of facultative gram negative rods. It is use to isolate gram negative anaerobes like bacteroides, prevotella, and some fusobacterium

  1. Anaerobic blood agar
  2. Anaerobic PEA blood agar
  3. Anaerobic kanamycin-vancomycin blood agar
  4. Anaerobic paromomycin-vancomycin laked blood agar (PVLB)
  5. Thioglycollate
  6. Bacteroides bile-esculin (BBE)
A

3

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15
Q

can inhibit the swarming of proteus and also inhibits the growth of facultative anaerobes such as the members of enterobacteriaceae. We can isolate gram positive and most gram negative obligate anaerobes

  1. Anaerobic blood agar
  2. Anaerobic PEA blood agar
  3. Anaerobic kanamycin-vancomycin blood agar
  4. Anaerobic paromomycin-vancomycin laked blood agar (PVLB)
  5. Thioglycollate
  6. Bacteroides bile-esculin (BBE)
A

2

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16
Q

Used to detect prevotella due to pigment production

  1. Anaerobic blood agar
  2. Anaerobic PEA blood agar
  3. Anaerobic kanamycin-vancomycin blood agar
  4. Anaerobic paromomycin-vancomycin laked blood agar (PVLB)
  5. Thioglycollate
  6. Bacteroides bile-esculin (BBE)
A

4

17
Q

detects organism that has the ability to grow in 20% bile and be able to hydrolyze esculin. Positive result for Esculin hydrolysis is brownblack color

  1. Anaerobic blood agar
  2. Anaerobic PEA blood agar
  3. Anaerobic kanamycin-vancomycin blood agar
  4. Anaerobic paromomycin-vancomycin laked blood agar (PVLB)
  5. Thioglycollate
  6. Bacteroides bile-esculin (BBE)
A

6

18
Q

is enriched broth with supplemented hemin and vitamin. It is use as a supplement to plating media or slow growing bacteria.

  1. Anaerobic blood agar
  2. Anaerobic PEA blood agar
  3. Anaerobic kanamycin-vancomycin blood agar
  4. Anaerobic paromomycin-vancomycin laked blood agar (PVLB)
  5. Thioglycollate
  6. Bacteroides bile-esculin (BBE)
A

5

19
Q

Used to demonstrate lecithinase activity

a. Egg yolk agar
b. Bile esculin
c. Thioglycollate

A

a

20
Q

Used to grow obligate and aerotolerant anaerobes

a. Egg yolk agar
b. Bile esculin
c. Thioglycollate

A

c

21
Q

Used to grow bacteroides

a. Egg yolk agar
b. Bile esculin
c. Thioglycollate

A

b

22
Q

Oxygen is removed from the system through reaction with hydrogen,
which has been added externally to the system. Reaction takes place in
the presence of palladium catalyst.

A. anaerobic Jar method
b. Evacuation-replacement system
c. Glove-Box method
d. Disposable anaerobic bags

A

a

23
Q

What is the indicator for anaerobic jar method?

a. Methylene blue strips
b. Resazurin dye

A

a

24
Q

In methylene blue strips, Color of methylene blue strip if oxygen is gone?

A

White

25
Q

In methylene blue strips, Color of methylene blue strip if oxygen remain?

A

Blue

26
Q

Indicator for Anaerobic jar method where the color of the indicator is pink (O2 present), white (absent)

a. Methylene blue strips
b. Resazurin dye

A

b

27
Q

The air in the jar is removed and replaced with a mixture of gas containing

a. 85% N2, 5% H2, and 10% CO2.
b. 85% N2, 5% H2, and 5% CO2.
c. 65% N2, 10% H2, and 5% CO2.
d. 85% N2, 10% H2, and 5% CO2.

A

d

28
Q

The air in the jar is removed and replaced with a mixture of gas containing 85% N2, 10% H2, and 5% CO2.

A. anaerobic Jar method
b. Evacuation-replacement system
c. Glove-Box method
d. Disposable anaerobic bags

A

b

29
Q

Redox indicator for Evacuation-replacement system?

A

Methylene blue strip

30
Q

Self-contained anaerobic environment of 85% N2, 10% H2, and 5% CO2 is provided through external gas sources

Useful for large laboratories that handle large volume of anaerobic
specimens

A. anaerobic Jar method
b. Evacuation-replacement system
c. Glove-Box method
d. Disposable anaerobic bags

A

c

31
Q

Plastic bags with a H2- CO2 gas generator that is released on addition of water

Can hold 1-3 petri dish)

A. anaerobic Jar method
b. Evacuation-replacement system
c. Glove-Box method
d. Disposable anaerobic bags

A

d

32
Q

Alternative of Anaerobic Jar method?

A

Candle jar

33
Q

This an anaerobic incubation sysem that is useful for large laboratorus that handles large volume of anaerobic samples

A

Anaerobic chambers

MTAP 1 Bacte (93 decks)

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