(Part 2) 2.20.7 Enterobacteriaceae Biochemical test and Media [part5]

Question 1

What is the original color of Triple Sugar Iron (TSI)?

Answer 1

ORange-Red

Question 2

What is the component of TSI in slant?

Answer 2

Lactose / Sucrose (1% each)

Question 3

What is the component of TSI in Butt?

Answer 3

Glucose (0.1%)

Question 4

What is the pH indicator for TSI?

a. Phenol red
b. Bromcresol Purple
c. Neutral red
d. Bromothymol blue

Answer 4

Phenol red

Question 5

What is the H2S indicator for TSI?

a. Ferrous ammonium citrate
b. Ferric Citrate
c. Ferrous Sulfate
d. Ferric ammonium Citrate

Answer 5

c

Question 6

The sulfur source for TSI is Sodium Thiosulfate

T or F

Answer 6

T

Question 7

indicator (+) for Gas in TSI

Answer 7

Cracks

Question 8

A/A + Gas indicates in TSI?

Answer 8

Acid over Acid with gas

Rapid lactose fermenter

Question 9

K/A indicates in TSI?

Answer 9

Alkaline over acid

Non lactose fermenter

Question 10

K/K indicates in TSI?

Answer 10

Alkaline over alkaline

Non glucose and Lactose fermenter

Question 11

Lysine iron agar (LIA) can be used to determine

• Deaminate lysine
• Decarboxylate lysine
• Produce H2S

T or F

Answer 11

T

Question 12

Original color of LIA?

Answer 12

Violet

Question 13

What is the pH indicator for LIA?

a. Phenol red
b. Bromcresol Purple
c. Neutral red
d. Bromothymol blue

Answer 13

b

Question 14

What is the H2S indicator for LIA?

a. Ferrous ammonium citrate
b. Ferric Citrate
c. Ferrous Sulfate
d. Ferric ammonium Citrate

Answer 14

a

Question 15

What is the Slant of LIA indicates?

Answer 15

Lysine Deamination

Question 16

What is the butt of LIA indicates

Answer 16

Lysine Decarboxylation

Question 17

K/K in LIA indicates?

Answer 17

Alkaline (-) over alkaline (+)

Question 18

R/A in LIA indicates?

Answer 18

Red (+) over Acid (-)

Question 19

NOTE:

In Butt of LIA, when there is decarboxylase

Purple -> Yellow (-) -> Purple (+)