Leukopoor Red Blood Cells

Question 1

What is the shelf life of leukopoor red blood cells in a closed system?

Answer 1

> The shelf life of leukopoor red blood cells in a closed system is the same as packed red blood cells.

Question 2

What is the shelf life of leukopoor red blood cells in an open system?

Answer 2

> Leukopoor red blood cells have a shelf life of 24 hours.

Question 3

How many Celcius in the storage temp for Leukopoor Red Blood Cells?

Answer 3

> 1-6ºC

Question 4

Give the contents about Leukopoor Red Blood Cells

Answer 4

> 5 x 106 residual White Blood Cell (WBC)

Question 5

Give the indications about Leukopoor Red Blood Cells

Answer 5

> Anemia with history of febrile reactions; to decrease alloimmunization to
WBC or HLA antigens or CMV transmission

Question 6

Enumerate the 3 means of Leukocyte Removal

Answer 6

1. Centrifugation
2. Washing procedures using saline or glycerol
3. Mechanical separation using leukoreduction filters

Question 7

Enumerate the 3 leukoreduction filters

Answer 7

1. First generation filters-170 um
2. Second generation filters-20-40 um
3. Third generation filters (3-log filter)

Question 8

Where does CMV reside in the body, and how does it typically manifest in healthy donors?

Answer 8

> CMV resides in WBC monocytes and macrophages and often manifests as a self-limiting virus, remaining asymptomatic among carriers who appear healthy.

Question 9

What is the method used for leukocyte reduction during centrifugation in an open system?

Answer 9

> Leukocyte reduction during centrifugation in an open system is achieved by selectively removing the buffy coat layer, which is rich in white blood cells.

Question 10

Washing procedures (open system) using __________ excess White Blood Cell (WBC) are removed

Answer 10

> Saline or glycerol

Question 11

Mechanical separation using __________ the best method in reducing the WBC population

Answer 11

> Leukoreduction filters

Question 12

Give the 3 leukoreduction filters.

Answer 12

1. First generation filters-170 um the one used
2. Second generation filters-20-40 um
3. Third generation filters (3-log filter)

Question 13

Enumerate the cells/components in the blood filtered in leukoreduction filters

Answer 13

1. Fibrin clot
2. Giant platelets
3. Microcytes- large red blood cells
4. Large white blood cells fragments
5. Most of the WBC

Question 14

Enumerate the cells pass through the filter

Answer 14

1. Normal platelets
2. Normal RBC
3. WBC same size with normal RBC

Question 15

Give the Centrifugation Procedure

Answer 15

1. Invert and Centrifuge
2. Remove the Red Cells

Leukocite-Poor Blood Storage 35 days at 4°C