Frozen< Thawed< Deglycerolized RBC

Question 1

Shelflife of FROZEN< THAWED< DEGLYCEROLIZED RBC and how many hours does Deglycerolized?

Answer 1

• Frozen – 10 years
• Deglycerolized – 24 hours

Question 2

How many Celcius in the storage temp for FROZEN< THAWED< DEGLYCEROLIZED RBC in Freezing time?

Answer 2

• 65ºC (High Glycerol-40%)
• 120ºC (Low Glycerol-20%)
• 65ºC (using 79%glycerol with dextrose fructose and EDTA)

Question 3

How many Celcius in Deglycerolizing Process

Answer 3

> 1-6ºC

Question 4

Why might FROZEN, THAWED, and DEGLYCEROLIZED RBCs be used, and what are their indications?

Answer 4

• Used for anemia treatment and long-term storage purposes.
• They are indicated for “rare” blood units and autologous units.

Question 5

Useful for Autologous donation transfusion which has rare phenotype such as Bombay negative

Answer 5

> FROZEN RBC- “GLYCEROLIZED / CRYOPROTECTED RBC”

Question 6

What is the purpose of freezing RBCs with glycerolization or cryoprotection?

Answer 6

> Freezing RBCs with glycerolization or cryoprotection is done to prolong their storage life.

Question 7

What are “GLYCEROLIZED / CRYOPROTECTED RBC” units, and how long is their viability and expiration date compared to regular donor blood units?

Answer 7

1. “GLYCEROLIZED / CRYOPROTECTED RBC” units, also known as Frozen RBCs, are red blood cells that have been preserved using glycerol and a cryoprotectant
2. These units can remain viable for up to 10 decades, giving them a much longer expiration date

Question 8

What is the process involved in creating “Frozen RBC -Glycerolized / Cryoprotected RBC”?

Answer 8

• By adding the first cryoprotective agent to the donor unit before storing the red blood cells (RBC) at an extremely low temperature.

Question 9

What is the purpose of using a penetrating cryoprotective agent that utilizes a low molecular weight agent?

Answer 9

• commonly employed in laboratory settings to prepare red blood cells (RBCs) before freezing.

Question 10

What is the most penetrating agent?

Answer 10

> Glycerol

Question 11

What are the benefits of using a penetrating agent in extreme cold/low-temperature storage for cells?

Answer 11

The penetrating agent serves several key functions:
1. Protects the cell membrane from extreme
cold/ low temp storage
2. Prevents ICE CYRSTAL FORMATION in the cell
3. Prevents the loss of water dehydration of
cell when in cold temperature

Question 12

How does the storage of a penetrating cryoprotective agent relate to the concentration of glycerol, and how long is it effective for?

Answer 12

• Depends on the concentration of glycerol. This storage method remains effective for up to one decade.

Question 13

How many percentage in high concentration glycerol more recommended because there is
no liquid nitrogen and the concentration is
not low?

Answer 13

> 40%

Question 14

Freezing req for RBC under high concentration glycerol __________ degrees which is mehanical freezer.

Answer 14

> 65 degrees

Question 15

How many percentage in low concentration glycerol under types of agents of cryoprotective RBC?

Answer 15

> 20% but there is a specific requirement of Liquid nitrogen
Very low concentration will result in cell lysis

Question 16

How many degrees does req for RBC under Low Concentration GLYCEROL?

Answer 16

> 120 degree

Question 17

What freezer does in Low concentration glycerol?

Answer 17

> Liquid nitrogen freezer

Question 18

What freezer does in high concentration glycerol?

Answer 18

> Mechanical Freezer

Question 19

What will happen if frozen RBC is needed?

Answer 19

> Thawing Process is Performed

Question 20

What is the process before the unit is transfused under Frozen RBC- “Glycerolized/Cryoprotected RBC”?

Answer 20

> Removed first the glycerol “DEGLYCEROL
PROCESS”= removal of glycerol in the donor unit

Question 21

Used washed solution which has 3 washed procedure
to removed glycerol from the unit.

Answer 21

> DEGLYCEROLIZING PROCESS

Question 22

Hypertonic saline solution in __________ until isotonic level is attained.

Answer 22

> Decreasing concentration

Question 23

How many hours does every after 3 washed placed the unit in ref temperature for
the meantime and transfused?

Answer 23

> Within 24 hrs

Question 24

Enumerate the 3 washed steps in deglycerolizing process.

Answer 24

1. WASHED THE THAWED with 12% saline solution which is hypertonic
   solution which removed the glycerol without affecting the viability of the
   RBC (It will not shrink it will maintain)
2. 2nd washed RBC with 1.6% saline solution “hypertonic” = facilitate the
   complete removal of glycerol without effecting the viability of RBC.
3. 3RD washed 0.2% dextrose in normal saline solution= Isotonic solution prior
   to transfusion which is add in NSS

Question 25

What is the process of Hypertonic solution in DEGLYCEROLIZING PROCESS?

Answer 25

> Remove glycerol to cell membrane

Question 26

What is the use of Physiologic solution Dextrose?

Answer 26

> it is used to provide additional source
of ATP of the cells

Question 27

What Normal Saline Solution-Isotonic maintaining of?

Answer 27

> Maintain the morphology of the cell prior to blood transfusion

Question 28

• High molecular weight agent
• “HES” HYDOXYL ETHYL STARCH- commonly used
• CAN’T PASS TRHOUGH THE CELL MEMBRANE

Answer 28

> 2nd- non penetrating cryoprotective agent

Question 29

In the 2nd non penetrating cryoprotecttive agent Sole Action protects ____________.

Answer 29

> Protects the cell surface from extremely low
temperature