1.4 immobilized enzymes Flashcards
1
Q
what are immobilised enzymes
A
immobilized enzymes are fixed bound or trapped on an inert matrix(or membrane)
∴ reducing frequency of collisions (substrate is the only moving molecule)
2
Q
where are immobilized enzymes used
A
(industrial)
- food
- pharmaceutical
- agrochemical
3
Q
what is an example of an immobilized enzyme
A
alginate beads
4
Q
advantages of immobalized enzymes:
A
- can be reused
- only a small enzyme quantity needed
- greater stability ∴ denature at higher temps
- no contamination
5
Q
disadvantages of immobilised enzymes
A
- cost a lot
- enzymes can become less active
6
Q
how does a biosensor that detects urea function
A
- urea molecules diffuse across semi-permeable membrane ∴ enzyme-substrate complex (immobilized urease)
7
Q
urea + urease =
A
ammonia
8
Q
what is a biosensor
A
analyse the concentration of substances in a solution
9
Q
A
