Microbiology Flashcards
BAP and CAP are examples of what media
Enriched / non-selective / nutritive
MAC is both _____ & _____ media
Differential and selective
What type of media is MSA
Selective media
Inhibit growth of other organisms while promoting growth of desired organism
Selective media
CM of Burkholderia pseduomallei
Ashdown medium
CM of Salmonella typhi
Bismuth sulfite agar
CM of Bordetella pertussis
Bordet-Gengou (potato blood glycerol)
CM of Legionella spp
BCYE (Buffered charcoal-yeast extract agar)
CM of Campylobacter spp.
Campy-blood agar
CM of Yersinia spp
CIN (Cefsulodin-irgasan-novobiocin agar)
CM of Pseudomonas aeruginosa
Cetrimide agar
CM of Bordetella perussis
Charcoal cephalexin blood agar
CM of Haemophilus spp. and Neisseria spp.
Chocolate agar
CM of Haemophilus spp.
Chocolate blood agar with horse blood
CM of Gram-positive cocci
CNA (Columbia colistin-nalidixic acid)
CM of Clostridium difficile
CCFA (Cyloserine-cefotixin fructose agar)
CM of Francisella tularensis
Cystine blood glucose agar
CM of Aspergillus spp.
Czapek’s agar
CM of Leptospira
Fletcher’s semi-solid medium
CM of Salmonella & Shigella vs. other enteric bacilli
Hektoen enteric (HE) agar
CM of Corynebacterium diphtheriae
Loeffler’s serum medium / Pai medium
CM of lactose vs. non-lactose fermenting enteric bacill
MacConkey agar
CM of E.coli 0157:H7
MacConkey sorbitol agar
CM of Staphylococci
Mannitol salt agar
CM of Mycobacteria
Middlebrook 7H10
Mueller Hinton Agar has a depth of
4 mm
CM of Bordetella pertussis
Regan-Lowe agar
CM of Campylobacter spp.
Skirrow agar
CM of N.gonorrhoeae and N. meningitis
Thayer-Martin agar
CM of anaerobes and aerobes
Thioglycollate broth
CM of Vibrio spp.
TCBS (thiosulfate citrate-bile salt sucrose)
CM of S. agalactiae
Todd-Hewitt broth
CM of Salmonella and Shigella
XLD (Xylose lysine desoxycholate) agar
Special stain for Mycolic acid (e.g. Mycobacteria)
Ziehl-Neelsen
Special stain for yeast cells
India ink (negative stain)
Special stain for endospores
Schaeffer-Fulton
Special stain for nucleic acid
Acridine orange
Special stain for flagella
Leifson
Special stain for polar bodies (Y. pestis)
Wayson
Special stain for Rickettsia
Gimenez stain
Special stain for Spirochetes
Dieterle silver stain
Special stain for Mycobacterium leprae
Fite-Faraco (counterstain: Hematoxylin)
Biosafety level: Equipped with handwashing sinks
1
Biosafety level: SARS-CoV-2, BSL 2
2
Biosafety level: Organisms are primarily transmitted by infectious AEROSOL, SARS-CoV-2, BSL-3
3
Biosafety level: EXOTIC AGENTS, maximum containment
4
Biosafety cabinet that filters EXHAUSTED air ONLY
1
70% recirculated 30% exhausted
2 (A1)
30% recirculated 70% exhausted
2 (B1)
BSC for handling SARS-CoV-2 testing
2 (A2)
100% exhausted NO recirculation
2 (B2)
BSC that is completely enclosed, centrifuge can be installed
3
HEPA
High-Efficiency Particulate Air
HEPA pore size
0-3 mm
Class 2 BSCs are also called
Vertical laminar flow BSCs
BSC for RADIOISOTOPES, toxic chemical and carcinogens
Class 2B
CSF specimen level of prioritization
1
For superficial abscess, swab along leading __________
Edge of the wound
Specimens for microbiology testing should be transported to the laboratory within
15 minutes (<15)
Draw blood at time of _____ episodes. How many sets
Febrile (before the fever spikes)
2 sets (left and right arms)
Incubate CSF at
37C
O & P examination. How many specimens
3 specimens
- every other day (outpatient)
- everyday (inpatient)
Transport stool in the laboratory within
2 hours
For sputum sample, gargle with
Water
Specimen of choice for Bordetella pertussis
Nasopharyngeal swab
Acetamide utilization is a test for __________ with a positive result of __________
Pseudomonas aeruginosa; blue color
Acetate utilization is a test for __________ with a positive result of __________
Escherichia coli; blue color
Bacitracin susceptibility is a test for __________ which uses _____ amount of bacitracin
Streptococcus pyogenes, 0.04 U
Bile esculin is a test for __________ with a positive result of __________
Enterococcus faecalis; blackening of agar slant
Bile solubility is a test for __________ with a positive result of __________
Streptococcus pneumonia; lysed colonies
Bile solubility test reagent to lyse the colony of S. pneumoniae
Sodium desoxycholate
Positive result for CAMP test
Arrowhead-shaped
In catalase test, 30% H2O2 will yield
H2O and O2