stan bio TPAG Flashcards

1
Q

Protein molecules contain a large number of __

A

peptide bonds

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2
Q

When treated with ___in alkaline solution, a
colored complex is formed between the copper and the carbonyl
and imine groups of these peptides

A

copper ions (Cu+2)

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3
Q

As a similar reaction occurs
with ___ (the simplest of such compounds formed by heating
urea),

the term “___ reaction” was adopted.

A

biuret

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4
Q

The method biuret reactio described is based on the reports of __

A

Weischselbaum and Gornal et al

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5
Q

The violet color developed is proportional to the number of ___ in the protein and is nearly independent of the relative concentration of albumin and globulin

A

peptide bonds

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6
Q

Reagents

A

Solution of copper sulfate pentahydrate - 0.3 g/dL,
in aqueous sodium hydroxide - 0.8 g/dL
potassium iodide and
potassium sodium tartrate

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7
Q

Total Protein Standard composition

A

Aqueous solution of Bovine Albumin, Fraction V, with sodium azide as preservative.

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8
Q

____ as preservative.

A

sodium azide

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9
Q

precaution:

Standard contains sodium azide as a preservative. May react with
____ to form explosive metal azide build up

A

copper or lead plumbing

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10
Q

Total Protein kit is stable at __ until the expiration date on the label.

A

2-8°C

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11
Q

Total Protein Reagent is stable at___.

A

2-30°C

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12
Q

Store standard at__.

A

2-8°C

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13
Q

Do not use ___, as fibrinogen gives increased results

A

plasma

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14
Q

Total protein in serum is reportedly stable for
____ at room temperature and for _____ at 2-8°C.

A

1 week; 30 days

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15
Q

This method is not to be used for the determination of
protein in ____

A

urine or spinal fluid

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16
Q

This method is not to be used for the determination of
protein in urine or spinal fluid, due to ___

A

low protein levels and high
concentrations of interfering compounds

17
Q

Reagent solution

A

1000 ul of reagent

18
Q

standard solution

A

1000 ul of reagent
10 ul of standard

19
Q

unknown solution

A

1000 ul of reagent
10 ul of sample

20
Q

absorbance wavelength

A

550 nm

21
Q

. Read S and U vs RB at 550 nm within how many mns or hrs?

A

within 1 hour

22
Q

formula

A

au/as X 10

23
Q
A