stan bio TPAG Flashcards
Protein molecules contain a large number of __
peptide bonds
When treated with ___in alkaline solution, a
colored complex is formed between the copper and the carbonyl
and imine groups of these peptides
copper ions (Cu+2)
As a similar reaction occurs
with ___ (the simplest of such compounds formed by heating
urea),
the term “___ reaction” was adopted.
biuret
The method biuret reactio described is based on the reports of __
Weischselbaum and Gornal et al
The violet color developed is proportional to the number of ___ in the protein and is nearly independent of the relative concentration of albumin and globulin
peptide bonds
Reagents
Solution of copper sulfate pentahydrate - 0.3 g/dL,
in aqueous sodium hydroxide - 0.8 g/dL
potassium iodide and
potassium sodium tartrate
Total Protein Standard composition
Aqueous solution of Bovine Albumin, Fraction V, with sodium azide as preservative.
____ as preservative.
sodium azide
precaution:
Standard contains sodium azide as a preservative. May react with
____ to form explosive metal azide build up
copper or lead plumbing
Total Protein kit is stable at __ until the expiration date on the label.
2-8°C
Total Protein Reagent is stable at___.
2-30°C
Store standard at__.
2-8°C
Do not use ___, as fibrinogen gives increased results
plasma
Total protein in serum is reportedly stable for
____ at room temperature and for _____ at 2-8°C.
1 week; 30 days
This method is not to be used for the determination of
protein in ____
urine or spinal fluid
