STAN BIO - BILIRUBIN Flashcards
Most routine clinical procedures for the determination of serum bilirubin
are based on the ___
classical Diazo reaction of Ehrlich
classical Diazo reaction of Ehrlich which was first applied to the estimation of this bile pigment in serum in what year and who are the people involved
1913 by Van den Berg and Snapper
An excellent review of many bilirubin methodologies
is given by __
Winkelman and his co-workers
When a solution of ___ in____ is combined
with one of ____, _____ is formed.
sulfanilic acid + dilute hydrochloric acid
sodium nitrite
= nitrous acid
This unstable acid, which must be freshly prepared for each group of assays, react to form ___
diazotized sulfanilic acid
diazotized sulfanilic acid also known as
p-benzenediazonium sulfonate”
p-benzenediazonium sulfonate couples with bilirubin to produce __
azobilirubin
the color
intensity of which is proportional to __.
bilirubin concentration
Since bilirubin standards suffer from a high degree of instability, the method presented utilizes a aqueous solution of ___ as a calibrator,
N-1naphthylethylenediamine dihydrochloride
the N-1naphthylethylenediamine dihydrochloride as a calibrator is according or made by
Bilissis and Speer
The calibrator supplied has been
prepared to yield, for the technique described, a color equivalent to the
labelled bilirubin level at what wavelength.
540 nm
: Total Bilibubin reagent, Direct Bilirubin
reagent, oxidant, and calibrator are stable when stored at _____ until the
expiration date on their respective labels
15-30°C
Discard oxidant if a ___ develops.
dark yellow color
An eventual color change of
Total Bilirubin reagent may occur but this color will not interfere with the
assay.
true or false
true
