[2] Class 6: Nucleotide Structure/ Metabolism Flashcards Preview

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Flashcards in [2] Class 6: Nucleotide Structure/ Metabolism Deck (66)
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1

DNA 5' to 3' gives:

Directionality

2

Which carbon of the ribose is deoxy in DNA:

2'

3

What type of bonds form between A-T and C-G

H bonds

4

DNA helix structure

R handed antiparallel 5'-3'

5

A-T how may h bonds?

2 h bonds

6

How many h bonds c-g

3 h bonds

7

Phosphodiester bond, where is it in dna?

Between 3' carbon of ribose and 5' methyl grp on other ribose (below)

8

Who observed nucleotides in DNA? Who received credit?

Rosalind franklin,

Watson and crick received the prize for it though

9

How many nucleotides in our genome?

3 billion

10

How is DNA condensed to fit in our cells?

DNA binding proteins (histones and non-histones) allows for dynamic state between condensation and rapid access for gene expression

11

Protein+Nuclear DNA=

Chromatin

12

Phosphate esters of nucleosides

Contain base, sugar and phosphate

Nucleotide

13

Base + sugar

Nucleoside

14

WHat type of bond is between the purine/pyramidine base and the ribose sugar?

Beta glycosidic linkage

15

Big ring:

Small ring:

Small name: purine

Big name: pyrimidine

16

Purine nucleotide base:

Nucleosides?

Adenine; guanine

Adenosine;guanosine

17

Pyramidine nucleotide base:

Nucleoside:

Cytosine, Uracil, thymine

Cytidine, uridine, thymidine

18

Difference in thymine and uracil?

2' methyl on thymine,

H on uracil

19

What are the other purines?

Hypoxanthine, inosine, xanthine, xanthosine

20

How can you go from a nucleotide to a DEOXYnucleotide?

Ribonucleotide reductase

21

What stimulates and inhibits ribonucleotide reductase?

ATP stimulates ribonucleotide reductase,

dATP nucleotides inhibits ribonucleotide reductase

22

Bonds of the 3 phosphates in ATP:

Ester bond on the alpha phosphate and 2 anhydride bonds on beta and gamma phosphates

23

4 specific roles of nucleotides:

ATP, CoA, NAD+, FAD

24

DNA bonded together is a phosphodiester bond: another name for this is

A covalent bond

25

DNA measurements:

Length between 1 full turn: 34å
10 basepairs /turn
Base pairs are separated by 3.4 å,
36º turn w/ each rotation of a new base pair.
20Å width of DNA

26

Why is DNA typically in B form: long and slender?

B/c it is typically hydrated in the cell

27

DNA is more stable w/ more o which base pair?

C-G b/c there are 3 H bonds

28

Catalyzes the formation of a phosphodiester bridge:

DNA polymerase

29

RNA of retrovirus is converted to DNA by what enzyme, and subsequent synthesis of DNA strand?

Reverse transcriptase

30

What enzyme is responsible for cutting long chain RNA/DNA to short oligomers?

(Deoxy)ribonucleases