2. Nucleic Acid Hybridization Theory Flashcards Preview

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Flashcards in 2. Nucleic Acid Hybridization Theory Deck (8)
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1

Probe-'patient' NA target complexes that form

Can be specific or non-specific

Heteroduplex

2

What happens when assay temp is ABOVE probe's Tm

Denatured state favoured (no hybridization)

3

Tm formula

Tm = 4(G+C) + 2(A+T)

4

What is melting temperature (Tm)

Temperature at which HALF of a nucleic acid duplex is ds and HALF is ss (i.e. half of a duplex is melted)

Always lower than Td

5

What is denaturation temperature (Td)

Temperature at which a double-stranded nucleic acid complex is COMPLETELY separated into single strands

Always higher than Tm

6

What happens when assay temp is BELOW probe's Tm

Specific AND non-specific hybridization occurs

7

Formed when complementary probe strands or complementary genomic DNA strands re-nature

Homoduplex

8

Ionic strength and temperature conditions (low/high) associated with HIGH stringency

LOW ionic strength

HIGH temperature