Flashcards in 7. Real-time PCR Deck (4)
DNA probe - what's on the 5' end and 3' end?
5' - reporter
3' - quencher
Why can't melt-curve analysis be done on TaqMan probes?
TaqMan probes are degraded for fluorescence detection (chewed up by Taq)
No probes to melt off of amplicons = can't get Tm info
In terms of real-time PCR, what is 'threshold'
Establishes the point where measurements are made - therefore placed somewhere in exponential phase of all amplification curves