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Flashcards in 4. NAT Pre-analysis Deck (4)
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1

Max storage time for whole blood at room temperature

Up to 24 hrs

2

Why should the buffy coat be separated from whole blood specimens if there will be a delay in NA isolation

Buffy coat will contain WBCs containing nucleic acids

If whole blood is frozen, cells will lyse upon thawing:

- hemoglobin co-isolating with DNA will inhibit PCR

- Lysed WBCs will have nucleic acids degraded by nucleases

3

Why is DNA isolated from heparin anti-coagulated blood not suitable for PCR

Heparin co-isolates with DNA during purification and it inhibits the activity of polymerase

4

Max storage time for whole blood at fridge temperature

Up to 72 hrs