4. NAT Pre-analysis Flashcards Preview

MDx 1 > 4. NAT Pre-analysis > Flashcards

Flashcards in 4. NAT Pre-analysis Deck (4)
Loading flashcards...

Max storage time for whole blood at room temperature

Up to 24 hrs


Why should the buffy coat be separated from whole blood specimens if there will be a delay in NA isolation

Buffy coat will contain WBCs containing nucleic acids

If whole blood is frozen, cells will lyse upon thawing:

- hemoglobin co-isolating with DNA will inhibit PCR

- Lysed WBCs will have nucleic acids degraded by nucleases


Why is DNA isolated from heparin anti-coagulated blood not suitable for PCR

Heparin co-isolates with DNA during purification and it inhibits the activity of polymerase


Max storage time for whole blood at fridge temperature

Up to 72 hrs