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Flashcards in 8. Isothermal NAAT Deck (5)
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1

What is HPA

Hybridization protection assay

2

TMA is an RNA amplification method driven by what 2 enzymes?

Reverse transcriptase

RNA polymerase

3

Reagent added to a completed TMA reaction to perform a Hybridization Protection Assay (HPA)

Acridinium ester (AE)-labeled DNA probes

4

How does isothermal amplification differ from PCR?

Target amplification occurs at a SINGLE temperature

5

Relative to PCR, how does TMA reduce the risk of false positives in subsequent assays?

TMA produces RNA amplicons

They're unstable outside reaction tube = low risk as a contaminant