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Flashcards in 3. Enzymes for NAT Deck (8)
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1

What is RT-PCR

Reverse transcriptase PCR

Where RNA first converted to DNA, then the DNA is amplified

2

Enzyme used in transcription-mediated amplification (TMA) and RT-PCR

Reverse transcriptase (RT)

3

4 components of a simple restriction digest in order of combining

1. Nuclease-free water

2. RE digest buffer

3. dsDNA to be cut

4. Restriction enzyme

4

RNA polymerase template requirements

dsDNA promoter sequence from which to initiate ssRNA synthesis

5

RNA polymerase is ___-directed and has ___ polymerase activity

DNA-directed

RNA polymerase activity

6

What is star activity

RE cuts at sequences similar to, but slightly different than, specific restriction sites

7

Taq has __' to __' polymerase function and __' to __' exonuclease function

5'-3' for both polymerase and exonuclease function

8

Reverse transcriptase is ___-directed and has ___ polymerase activity

RNA-directed

DNA polymerase activity