25. Family Leptospiraceae Flashcards Preview

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Flashcards in 25. Family Leptospiraceae Deck (31):
1

Causative agent of Lyme disease

Borrelia burgdorferi

2

Causative agent of relapsing fever

Borrelia hermsii

3

Causative agent of Syphilis

Treponema pallidum

4

Disease caused by other treponemes other than T. pallidum

Yaws
Pinta
Bejel

5

Genera under the Family Leptospiraceae

Borrelia
Leptospira
Treponema

6

Microscopic appearance of Leptospira spp.

Coiled rods with hook

7

Oxygen requirements of Leptospira spp.

Obligate aerobes

8

Specimens cultured for Leptospirosis diagnosis

Blood (early)
Urine (late, >2 weeks)

9

Molecular diagnosis of Leptospirosis

PCR

10

Serological diagnosis of Leptospirosis

Enzyme immunoassay (EIA)

11

Oxygen requirements of Borrelia spp.

Microaerophilic

12

Optimum temperature for growth of Borrelia

28-30°C

13

Determines the helical shape of Borrelia spp.

Flagella

Flagella-negative mutants are straight bacilli

14

Disease caused by B. burgdorferi

Lyme borreliosis

15

Vectors for Lyme borreliosis

- Ixodes scapularis: deer tick/black-legged tick
- Ixodes pacificus

16

Stages of Lyme disease

Stage 1 (3-30 days)
- 75% develop erythema chronicum migrans

Stage 2 (1-7 months)
- cardiac and neurologic problems

Stage 3 (months to years)
- arthritis

17

Explain the Western Blot diagnosis of Lyme borreliosis

POS IgM: 2 of 3 protein bands
POS IgG: 5 of 10 protein bands

18

Disease caused by T. pallidum

Syphilis

19

MOT of T. pallidum

Direct contact with lesions that contain T. pallidum

20

Stages of syphilis

Primary: chancre
Secondary: rash
Latent
Tertiary: gummas/neurosyphilis

21

Diagnostic test for primary syphilis

Dark-field microscopy

22

Stages of syphilis when it is infectious

Primary
Secondary
Latent (early)

23

Diagnostic tests for secondary syphilis

Dark-field microscopy (early)
Serological tests

24

Diagnosis of tertiary syphilis

Serological tests

25

In utero infection of T. pallidum

Congenital syphilis

26

Manifestations of tertiary syphilis

- gummatous syphilis
- cardiovascular disease
- neurosyphilis

27

Examples of non-treponemal tests

RPR
VDRL

28

Examples of treponemal tests

TP-PA
EIA

29

Stain for all spirochetes

Silver

30

Stain for Borrelia only

Giemsa

31

Causative agent of leptospirosis

Leptospira interrogans