1. Intro (II) Flashcards Preview

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Flashcards in 1. Intro (II) Deck (24):
1

Fermentable sugars in HEA

Lactose
Sucrose

2

pH indicators in HEA

Bromophenol blue
Acid fuchsin

3

Component of HEA; detects H2S production

Ferric ammonium citrate

4

HEA is differential for

Salmonella and Shigella

5

Characteristics of lactose fermenters in HEA

Yellow-orange colonies (E. coli)

6

Characteristics of non-lactose fermenters in HEA

Colorless/green colonies with unchanged medium (Shigella, Providencia)

7

Characteristics of H2S producers in HEA

Black colonies (Salmonella)

8

CNA inhibits

Most gram-negative organisms

9

Purpose of phenylethyl alcohol in PEA

Inhibits gram-negative organisms

10

3 key media for routine anaerobic organisms

Blood agar
Supplemented PEA
Kanamycin-vancomycin-laked blood agar (K-V agar)

11

Usual agar base for Blood Agar

Trypticase soy
Brain heart infusion

12

A good nonselective medium for initial isolation of anaerobes

Blood Agar

13

Agar that is selective for anaerobes

Supplemented PEA

14

2 components added to PEA to make it selective for anaerobes

Vitamin K
Hemin

15

Function of Vitamin K and hemin in Supplemented PEA

Suppresses growth of facultative gram-negative bacilli

16

Agar that is selective for gram-negative anaerobic bacilli

Kanamycin-Vancomycin-laked blood agar (K-V agar)

17

Component of K-V agar that helps separate aerobes and anaerobes in mixed cultures

Aminoglycoside

18

Components of K-V agar that permits growth of only gram-negative anaerobes

Kanamycin and Vancomycin

19

Agar that limits the swarming of Proteus spp.

K-V agar

20

Broth medium that serves as a check for plates when the primary shows no growth

Back-up Broth

21

Use of back-up broth should be limited to ______ to _______

Fluids
Tissue

22

Atmospheric requirements for microaerophilic organisms

5% O2
10% CO2
85% N

23

Atmospheric requirements for anaerobes

85% N2
10% H2
5% CO2

24

Component of HEA that inhibits gram-positive organisms

Bile salts