9. Family Enterobacteriaceae (I) Flashcards Preview

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Flashcards in 9. Family Enterobacteriaceae (I) Deck (55):
1

Microscopic appearance of the family Enterobacteriaceae

Gram-negative bacilli

2

Oxygen requirements for the family Enterobacteriaceae

Facultative anaerobes

3

Colony morphology for most Enterobacteriaceae

Large
Gray
Spreading colonies

4

2 genera of the family Enterobacteriaceae that generally differs in colony morphology

Klebsiella
Enterobacter

5

Colony morphology of Klebsiella and Enterobacter

Mucoid

6

Carbohydrate fermented by all Enterobacteriaceae

Glucose

7

Nitrate reduction characteristics of Enterobacteriaceae

Reduces nitrates to nitrites (with rare exceptions)

8

Oxidase reaction of Enterobacteriaceae

Oxidase negative

9

Motility of Enterobacteriaceae

Most are motile by peritrichous flagella

10

Cell-associated antigens of the family Enterobacteriaceae

O: somatic antigen (heat stable)
K: capsular antigen (heat labile)
H: flagellar antigen (heat labile)

11

Cell-associated antigen associated with endotoxin release

O

12

Which cell-associated antigen when present, causes the strains to be more pathogenic?

K

13

Cell-associated antigen responsible for motility

H

14

Which cell-associated antigen(s) is/are heat stable?

O

15

Which cell-associated antigen(s) is/are heat labile?

K
H

16

Which of the Enterobacteriaceae are primary intestinal pathogens?

Plesiomonas
Salmonella
Shigella
Yersinia enterocolitica

PSSY

17

Which of the Enterobacteriaceae are opportunistic?

Proteus
Providencia
Escherichia
Morganella
Citrobacter
Hafnia
Edwardsiella
Enterobacter
Klebsiella
Serratia

PPEMCHEEKS

18

Media used to recover Enterobacteriaceae

MacConkey agar
XLD
Hektoen

19

Carbohydrate used for initial differentiation of Enterobacteriaceae

Lactose

20

Food-borne enteric pathogens

Salmonella
E. coli
Campylobacter
Yersinia

SECY

21

Human to human enteric pathogens

Shigella
Salmonella typhi
Helicobacter pylori

SSH

22

Water-borne enteric pathogens

Vibrio
Aeromonas
Plesiomonas

VAP

23

Salmonella heat sensitive antigens for serotyping

H antigens

24

Salmonella virulence antigens for serotyping

Vi (K) antigens

25

Salmonella antigens that may mask O antigens

Vi (K) antigens

26

How to remove Vi (K) antigens so that it will not mask O antigens?

Boiling

27

Most common illness caused by Salmonella

Gastroenteritis

28

Salmonella spp. that causes bacteremia/septicemia

Salmonella choleraesuis

29

Disease caused by S. typhi

Enteric fever: Typhoid fever

30

Culture(s) used to diagnose S. typhi infection

Blood culture: + during first week
Stool culture: + during second week

31

Culture(s) used to diagnose gastroenteritis caused by Salmonella

Positive stool cultures

32

Culture used to diagnose infection with S. choleraesuis

Positive blood culture

33

Disease caused by S. choleraesuis

Bacteremia/septicemia

34

Serotyping of Shigella species based on O antigen

A: S. dysenteriae
B: S. flexneri
C: S. boydii
D: S. Sonnei

35

Which Shigella spp. are physiologically similar?

S. dysenteriae (A)
S. flexneri (B)
S. boydii (C)

36

How do you differentiate S. sonnei (Group D) from Groups A-C?

Biochemical tests

37

MOT of Shigella spp.

Fecal-oral route (human to human)

38

True/False. Shigella spp. are vulnerable to gastric acidity.

False. Shigella spp. are resistant to gastric acids

39

Toxin by Shigella spp. that causes inflammation and ulcerative lesions

Shiga toxin (cytotoxin)

40

Lactose ferementation characterictics of Shigella spp.

Lactose negative

41

TSI characteristics of Shigella spp.

Alkaline/acid
No gas
No H2S

42

Urease characteristics of Shigella spp.

Urease negative

43

Most important E. coli

Enterohemorrhagic E. coli (EHEC)

44

Enterohemorrhagic E. coli is also known as

E. coli O157:H7

45

Carbohydrates fermented by E. coli

Glucose
Lactose
Xylose

46

Indole reaction of E. coli

Indole positive

47

Methyl Red (MR) reaction of E. coli

MR positive

48

H2S production of E. coli

No H2S

49

Urease production of E. coli

No urease

50

Citrate reaction of E. coli

Citrate negative

51

Media used for E. coli O157:H7

MacConkey with Sorbitol (SMAC)

52

Colony morphology of E. coli on SMAC

Clear (does not ferment sorbitol)

53

Lactose fermentation characteristics of Salmonella

Lactose negative

54

H2S production of Salmonella

H2S positive

55

The family Enterobacteriaceae is normal flora in the

GI tract