Flashcards in 9. Family Enterobacteriaceae (I) Deck (55):
Microscopic appearance of the family Enterobacteriaceae
Oxygen requirements for the family Enterobacteriaceae
Colony morphology for most Enterobacteriaceae
2 genera of the family Enterobacteriaceae that generally differs in colony morphology
Colony morphology of Klebsiella and Enterobacter
Carbohydrate fermented by all Enterobacteriaceae
Nitrate reduction characteristics of Enterobacteriaceae
Reduces nitrates to nitrites (with rare exceptions)
Oxidase reaction of Enterobacteriaceae
Motility of Enterobacteriaceae
Most are motile by peritrichous flagella
Cell-associated antigens of the family Enterobacteriaceae
O: somatic antigen (heat stable)
K: capsular antigen (heat labile)
H: flagellar antigen (heat labile)
Cell-associated antigen associated with endotoxin release
Which cell-associated antigen when present, causes the strains to be more pathogenic?
Cell-associated antigen responsible for motility
Which cell-associated antigen(s) is/are heat stable?
Which cell-associated antigen(s) is/are heat labile?
Which of the Enterobacteriaceae are primary intestinal pathogens?
Which of the Enterobacteriaceae are opportunistic?
Media used to recover Enterobacteriaceae
Carbohydrate used for initial differentiation of Enterobacteriaceae
Food-borne enteric pathogens
Human to human enteric pathogens
Water-borne enteric pathogens
Salmonella heat sensitive antigens for serotyping
Salmonella virulence antigens for serotyping
Vi (K) antigens
Salmonella antigens that may mask O antigens
Vi (K) antigens
How to remove Vi (K) antigens so that it will not mask O antigens?
Most common illness caused by Salmonella
Salmonella spp. that causes bacteremia/septicemia
Disease caused by S. typhi
Enteric fever: Typhoid fever
Culture(s) used to diagnose S. typhi infection
Blood culture: + during first week
Stool culture: + during second week
Culture(s) used to diagnose gastroenteritis caused by Salmonella
Positive stool cultures
Culture used to diagnose infection with S. choleraesuis
Positive blood culture
Disease caused by S. choleraesuis
Serotyping of Shigella species based on O antigen
A: S. dysenteriae
B: S. flexneri
C: S. boydii
D: S. Sonnei
Which Shigella spp. are physiologically similar?
S. dysenteriae (A)
S. flexneri (B)
S. boydii (C)
How do you differentiate S. sonnei (Group D) from Groups A-C?
MOT of Shigella spp.
Fecal-oral route (human to human)
True/False. Shigella spp. are vulnerable to gastric acidity.
False. Shigella spp. are resistant to gastric acids
Toxin by Shigella spp. that causes inflammation and ulcerative lesions
Shiga toxin (cytotoxin)
Lactose ferementation characterictics of Shigella spp.
TSI characteristics of Shigella spp.
Urease characteristics of Shigella spp.
Most important E. coli
Enterohemorrhagic E. coli (EHEC)
Enterohemorrhagic E. coli is also known as
E. coli O157:H7
Carbohydrates fermented by E. coli
Indole reaction of E. coli
Methyl Red (MR) reaction of E. coli
H2S production of E. coli
Urease production of E. coli
Citrate reaction of E. coli
Media used for E. coli O157:H7
MacConkey with Sorbitol (SMAC)
Colony morphology of E. coli on SMAC
Clear (does not ferment sorbitol)
Lactose fermentation characteristics of Salmonella
H2S production of Salmonella