3B and 3C Flashcards

(38 cards)

1
Q

Rosalind Franklin

A

the structure of DNA - Franklin’s X-ray and creation of the Photo of the double-helix structure

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2
Q

Watson & Crick

A

proposed the double helix structure of DNA by building it. stole franklin’s light

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3
Q

DNA function

A

store and transmit genetic information, acting as the blueprint for all living organisms and viruses.

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4
Q

Chargaff’s Rules

A

A=T and G=C

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5
Q

DNA structure

A

a double-stranded molecule with a twisted ladder shape known as a double helix. Dna is a nucleic acid made up of nucleotides

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6
Q

bases of DNA vs RNA

A

Adenine, thymine, guanine, cytosine vs Adenine, uracil, guanine, cytosine

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7
Q

Purpose of DNA replication

A

to produce two identical copies of a DNA molecule,so each new cell receives a complete set of genetic information

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8
Q

replication fork

A

Y-shaped structure formed during DNA replication where the double-stranded DNA helix is separated

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9
Q

Topoisomerase

A

enzymes that unwinds dna BUT DOSENT SEPERATE for helicase to follow

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10
Q

DNA helicase

A

an enzyme that unzips the double-stranded DNA helix, separating the two strands

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11
Q

DNA polymerase

A

enzymes that create DNA molecules by assembling nucleotides

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12
Q

DNA ligase

A

enzymes that fills the junctions between okazaki fragments and primer

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13
Q

RNA primer

A

short stretches of RNA that provide a starting point for DNA polymerase to begin synthesizing a new DNA strand

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14
Q

Okazaki fragment

A

short, newly synthesized DNA sequences on the lagging strand during DNA replication formed by polymerase

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15
Q

leading vs lagging strand

A

the leading strand is synthesized continuously and goes from 3’ to 5’, while the lagging strand is synthesized in short, discontinuous fragments called Okazaki fragments going from 5’ to 3’

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16
Q

Similarities and differences between RNA & DNA

A

DNA is double-stranded, utilizes deoxyribose sugar, and contains thymine, while RNA is single-stranded, uses ribose sugar, and contains uracil instead of thymine

17
Q

mRNA

A

carries genetic info from nucleus to ribosome

18
Q

Trna

A

serves as a link between the mRNA and the growing chain of amino acids that make up a protein.

19
Q

rRNA

A

form ribosome and catalyzing the formation of peptide bonds between amino acids

20
Q

intron

A

non coding sequences that are removed

21
Q

exons

A

a segment of a DNA or RNA molecule containing information coding for a protein or peptide sequence.

22
Q

RNA splicing/editing

A

processes that modify pre RNA transcripts by removing introns. after transcription but still in the nucleus

23
Q

Substitution mutation

A

change in a single nucleotide base in a DNA sequence. ONLY A GENE/POINT mutation

24
Q

Insertion mutation

A

one or more nucleotide base pairs are added into the DNA sequence. could result to frameshift.ONLY A GENE/POINT mutation

25
Deletion mutation (gene)
the removal of a portion of the genetic material. could result to frameshift. ONLY A GENE/POINT mutation
26
Gene mutations vs chromosomal mutations
Gene mutations alter the DNA sequence within a single gene, while chromosomal mutations involve changes in the structure or number of chromosomes.
27
promoter
specific DNA sequence that signals the start of a gene's transcription. It acts as a binding site for RNA polymerase and other proteins, initiating the process of copying the gene's information into RNA.
28
Frederick griffith
wanted to know how bacteria causes disease. Injected mice with disease causing bacteria, heat killed bacteria, a mixture of harmless and heat killed, and harmless bacteria. Griffith learned that genetic information (DNA) can be transferred from one bacterial strand to another
29
transformation
the process where a cell takes in new DNA either by scientist or environment
30
Oswald avery
wanted to know which molecule in the heat killed bacteria was most important for transformation. Avery extracted molecules from the heat killed bacteria and combined with enzymes but transformation still occurred. they continued this to realize DNA was the transforming factor NOT PROTEIN. Avery discovered DNA stores and transmits gene info from one generation of bacteria to the next.
31
Hershey Chase
used bacteriophages in order to determine whether genes were made of proteins or DNA. showed that when bacteriophages infect bacteria, their DNA enters the host bacterial cell, but most of their protein does not
32
bacteriophages
type of virus that infects bacteria
33
transcription
In nucleus, segments of Dna template (3' to 5') serve as templates to produce complementary RNA (5' to 3') 1) polymerase binds to promoter and separates DNA 2) polymerase moves along template strand and creates rna 3) Polymerase goes until termination sequence and then detaches
34
template strand
the strand of DNA that is being read to create mRNA
35
deletion (chromosome)
the loss of one or more section from a chromosome. ONLY IN CHROMOSOMAL
36
Duplication
the addition/ duplication of a segment within a chromosome. ONLY IN CHROMOSOMAL
37
inversion
The swap of two segments within a chromosome. (they switch places) ONLY IN CHROMOSOMAL
38
translocation
a piece of one chromosome breaks off and attaches to another chromosome, or where pieces from two different chromosomes swap places. ONLY IN CHROMOSOMAL.