9-13

Question 1

starts the assembly process

Answer 1

TBP of TFIID

Question 2

PIC

Answer 2

everything there but no open complex formed yet

Question 3

what triggers the synth of RNA

Answer 3

entry of NTP

Question 4

plays a role in the regulation of initiation and is a cofactor that suports regulation of pol II tracription

Answer 4

TFIIA

Question 5

mutations in what resutl in xeroderma pigmentosum

Answer 5

XPD and XPB

Question 6

mutations in what results in trichothiodystrophy group A

Answer 6

TTDA of TFIIH

Question 7

what binds to enhancer sequences

Answer 7

tractiptional activatior proteins and assocaited coactivators

Question 8

signals the correct order of subsequent mRNA processing

Answer 8

serine phosphorylation of rbp1

Question 9

first major modification of eukaryotic mRNA

Answer 9

capping

Question 10

when is the fifth serine in rbp1 phosphorylated

Answer 10

as rna pol II clears the promoter region

Question 11

capping of first two reactions is done by a bifunctional enzyme in what

Answer 11

mammals

Question 12

uses 3 diff enzymes for capping

Answer 12

yeast

Question 13

second major modification in eukaryotic mRNA

Answer 13

splicing

Question 14

intervening sequences

Answer 14

introns

Question 15

excpetions to the fact that introns dont cotain genes and are degraded after excision

Answer 15

snoRNA and miRNA and group II introns encoding for maturases

Question 16

type of reaction for removal of introns

Answer 16

transesterfictaion reactions SN2

Question 17

how are splice sites defined

Answer 17

3D structure
positions ends of exons close together
conserved GU wobble pair

Question 18

what is the nuc of the branch point adenosien

Answer 18

2’ OH

Question 19

what was used to show branch point adenosine had 3 phosphate groups

Answer 19

RNaseT2

Question 20

how are intron splice sites defined

Answer 20

four short sequence motifs
1. GU 5’
2. AG 3’
3. BRANCH POINT ADENOSINE
4. polypyrimidine region upstream of 3’ splice sitewhat is

Question 21

what do other conserved bases in the exon intron boundaries do

Answer 21

produce diferent mature mRNAs from same gene and defines the exon intron boundary

Question 22

what uses U2 and U12

Answer 22

verterbates

Question 23

what is pressnt in ATAC introns

Answer 23

AU A AC

Question 24

how big are snRNAs

Answer 24

100-300

Question 25

what are the smith antigen

Answer 25

the proteins in snRNPs that the antibodies she produced

Question 26

what does U6 bind with

Answer 26

8 LSm protiens

Question 27

activated spliceosome

Answer 27

complex B act

Question 28

what is intially bound to the 3' end of the intron

Answer 28

non-snRNP proteins BBP U2AF

Question 29

what cuases the adenosine to be exposed and ready for catalysis

Answer 29

U2 mismathc with branch onit

Question 30

what organizes the tri snRNP

Answer 30

Prp8 protein

Question 31

when si the branch point adenosine in the right place

Answer 31

in complex B*

Question 32

what happens before complex c can form

Answer 32

inhibitory proteins must dissociate allwing active site to form

Question 33

holds the ends of the 5' and 3' exons

Answer 33

U5

Question 34

protein complexes that mark the RNA at site of splicing reaction

Answer 34

EJC

Question 35

helps recognize premature terination codons

Answer 35

EJC

Question 36

how is splice site choice regulated

Answer 36

Trans acting spliicing factors that bind to SRE's

Question 37

what do trans acting factors do

Answer 37

promote or inhibit recognition of nearby splice sites affect the functioning of U1 and U2

Question 38

promote splicing

Answer 38

SR rich proteins

Question 39

inhibtit splicing

Answer 39

hnRNPs

Question 40

context dependent splicing

Answer 40

NOVA binding to YCAY motif in exon- ESE upstream intron- ISS downstream intron- ISE

Question 41

mutation of splicing

Answer 41

COLQ holds acetylcholinesterase SRSF1 binds to GGAGG in exon 16 and enhances U1 to nearby splice cite hnRNP binds to mutated GGGGG in exon 16 causing it to be skipped

Question 42

mutation in this results in congential myasthenic syndrome

Answer 42

COLQ

Question 43

less common in animasl and rare in plants but 95% in humans

Answer 43

alternate splicing