extra

Question 1

what does CCA accept

Answer 1

CTP OR ATP

Question 2

CCA and unstable tRNA

Answer 2

targets them for degradation

Question 3

hypermodified purine- 3’ end of 5’ end

Answer 3

3’ end

Question 4

wobble position 3’ or 5’

Answer 4

5’ end

Question 5

what are some non watson crick interaction

Answer 5

G:U found in the wobble position

Question 6

what type of rxn is the aminoacylation reaction

Answer 6

SN2

Question 7

between what does the SN2 reaction occur

Answer 7

between the tRNA and the adenylated AA

Question 8

what remians bound to the aaRS

Answer 8

the aminoacyladenylate

Question 9

what class of aaRS binds to the anitcodon

Answer 9

class I

Question 10

tRNAala structural feature

Answer 10

G3:U70 and ONLY THAT

Question 11

what happens after the likely AA is selected

Answer 11

THEN it is proof read

Question 12

what are the two different sites in aaRS

Answer 12

aminoacylation and editing sites

Question 13

when is an AA kicked out from the small seive

Answer 13

if it fits in the editing site

Question 14

what is chekced in editing pretransfer

Answer 14

aminoacyl adenylate

Question 15

what is checked in editing posttransfer

Answer 15

the aatRNA

Question 16

chapeville experiment

Answer 16

raney nickel experiment

Question 17

what is missing glutamine and aspargine aaRS

Answer 17

bacteria and archaea

Question 18

what desulfurases cytein

Answer 18

cysteine desulfurase

Question 19

what uses formyl methionine

Answer 19

only bacteria

Question 20

what enzyme adds a formly group

Answer 20

transformylase

Question 21

what prevents EFTu and eEF1A from binding to tRNA

Answer 21

structural antideterminants on initiator tRNAs
but normal tRNAs do bind to these factors

Question 22

what are the structural determinants

Answer 22

bacterial- CA wobble in the acceptor step

euk- AU pair in acceor stem

both hace three GC in the anticodon stem

Question 23

structure of shine delgarno sequence and consensus sequence

Answer 23

purine rich

AGGAGG

Question 24

what serves as a timing mechanism

Answer 24

GTPase activity

Question 25

what accelerates the rate of 30s and 50s subunits joining

Answer 25

IF2

Question 26

what does the 70s formation lead to

Answer 26

GTP hydrolyssi sby IF2 which drives a series of maturation steps that enable the 70s to enter the elongation stage of protein synth

Question 27

what does GTP hydrolysis lead to

Answer 27

conformational changes that are linked with orderly progression of the ribosome through translation

Question 28

what do antibiotics bind to

Answer 28

critical regions of the ribosome or translation factors

Question 29

what does kasugamycin do

Answer 29

indirectly induces dissociation of P site bound FmettRNAfmet from 30s subunit through perturbation of mRNA