AMS

Question 1

What is the systematic name of amylase?

Answer 1

E.C.3.2.1.1

Question 2

What is the chemical name of AMS?

Answer 2

a-1,4-glucan-4-glucanohydrolase

Question 3

What does the amylase hydrolyze?

Answer 3

1,4-a-glucocidic linkage in
polysaccharides.

Question 4

The only enzyme that can be found in the urine

Answer 4

Amylase

Question 5

What is the MW of amylase?

Answer 5

45,000

Question 6

What is the optimum pH of Amylase?

Answer 6

Optimum pH: 6.9 – 7.0 in serum

Question 7

Without this, amylase cannot perform its duty.

Answer 7

Calcium

Question 8

Where is the amylase secreted in the pancreas?

Answer 8

Acinar cells

Question 9

Responsible in the hydrolysis of starch from the food
that we eat.

Answer 9

Salivary gland

Question 10

Small amount of Amylase can be found

Answer 10

SEMINAL FLUID, TESTIS, OVARIES, FALLOPIAN TUBES, STRIATED
MUSCLE, LUNGS AND ADIPOSE TISSUE

Question 11

first milk produced by a lactating
mother

Answer 11

Colostrum

Question 12

it hydrolyses the 1,4-a-glucocidic
linkage to form the following 3 products:

Answer 12

a. Glucose
b. Maltose
c. Dextrin

Question 13

What are the reactions catalyzed by amylase?

Answer 13

Starch digestion
Splits complex CHO made up of a-D glucose units

Question 14

 Inhibited by monoclonal antibody
 Inhibited by protein isolated from wheat
 Accounts for the 2/3 of the Amylase activity in
the normal serum

Answer 14

S-type Isoamylase

Question 15

Fastest migrating in electrophoresis

Answer 15

S1 isoamylase

Question 16

 Inhibited by monoclonal antibody
 Not inhibited by protein isolated from wheat

Answer 16

P-type Isoamylase (Pancreas)

Question 17

What is the most common fraction type of isoenzyme?

Answer 17

P2, S1 AND S2

Question 18

What p-type isoenzyme is predominant in cases of acute pancreatitis?

Answer 18

P3

Question 19

Abnormal amylase in combination with Immunoglobulins
(IgA or IgG) or other high MW proteins can also bind to Stype isoenzyme

Answer 19

Macroamylase

Question 20

What specific immunoglobulins do abnormal amylase combine to?

Answer 20

IgA OR IgG

Question 21

Sudden inflammation of the pancreas causing
abdominal pain.

Answer 21

Acute pancreatitis

Question 22

Predisposal for acute pancreatitis

Answer 22

Alcoholism

Question 23

Cause of ACUTE PANCREATITIS?

Answer 23

Gallstone formation

Question 24

Viral infection (Paramyxoviridae) that mainly
effect the parotid gland and the salivary gland

Answer 24

Mumps

Question 25

Two-folds increase

Answer 25

Perforated peptic ulcer

Question 26

Three-folds increase

Answer 26

Appendicitis

Question 27

Mimics acute pancreatitis

Answer 27

Ruptured ectopic pregnancy

Question 28

 Tear in the inner layer of the aorta took place  Mimic acute pancreatitis

Answer 28

Dissecting aortic aneurysm

Question 29

Example of billiary tract disease.

Answer 29

Cholecystitis

Question 30

Four-folds increase in the ULN of Amylase

Answer 30

Billiary tract disease

Question 31

Improved the reaction stoichiometry – employ the use of small oligosaccharide as a substrate.

Answer 31

CONTINUOS MONITORING METHOD

Question 32

more controlled and consistent hydrolysis conditions

Answer 32

CONTINUOS MONITORING METHOD

Question 33

Saccharogenic is by?

Answer 33

Nelson Somoyogi modification by Henry and Chiamon

Question 34

- Uses starch as a substrate - It measures the appearance of products

Answer 34

Saccharogenic

Question 35

Hydrolysis of starch by amylase causes a decrease in color intensity

Answer 35

Amyloclastic (iodometric method)

Question 36

Starch attached to chromogenic dye to form an insoluble dye substrate complex.

Answer 36

Chromogenic (Klein, Foreman, Searcy)

Question 37

Counterpart of Amyloclastic because we are looking at the color intensity (pagkaroon ng kulay)

Answer 37

Chromogenic (Klein, Foreman, Searcy)

Question 38

Measures the change in turbidity of starch solution over a short reaction period.

Answer 38

Turbidimetry and Nephelometry (Peralta and Reinhart)

Question 39

waste product of normal/physiological muscle metabolism

Answer 39

Creatinine

Question 40

Normal amylase/ creatinine ratio:

Answer 40

1.0% - 4.0% (0.01 – 0.04)

Question 41

A:C ratio (Acute pancreatitis) =

Answer 41

>4.0% (Up to15%)

Question 42

Enumerate the sources of amylase

Answer 42

Pancreas Salivary gland

Question 43

Enumerate the most effective activators of amylase

Answer 43

Bromide Chloride

Question 44

HISTORICAL METHOD OF AMYLASE

Answer 44

Saccharogenic Amyloclastic (Iodometric method) Chromogenic (Klein, Foreman, Searcy) Turbidimetry and Nephelometry (Peralta and Reinhart)