LESSON 2

Question 1

This way of naming the enzymes is formulated by IUB and IUPAC.

Answer 1

Standard system

Question 2

Transcribe IUPAC.

Answer 2

International Union of Pure and Applied Chemistry

Question 3

This type of naming the enzyme describes the nature of the reaction catalyzed and uses numerical code designation prefixed with the letter “E.C.”

Answer 3

Systematic Name

Question 4

What does “E.C.” stands for?

Answer 4

Enzyme Commission

Question 5

If E.C. 3.1.3.1: ALP. How about for ACP?

Answer 5

E.C.3.1.3.2

Question 6

What is the purpose of using the Numerical Code Designation a.k.a E.C. numbers?

Answer 6

To remove all ambiguity about the enzyme’s identity.

Question 7

What is the systematic name of LDH?

Answer 7

E.C.1.1.1.27

Question 8

What digit represents this: “Sub-class: acting on primary and secondary alcohols”

Answer 8

2nd digit

Question 9

This type of naming is a non-specific, practical and working name. It also used acronyms and abbreviations. What is this?

Answer 9

Trivial name

Question 10

What is the example of Trivial name?

Answer 10

Serum glutamic oxaloacetic transaminase
Serum glutamate pyruvate transaminase

Question 11

This is a multichained enzymes of similar activity and appear in different tissue, organ and cell organelle of similar organisms.

Answer 11

Isoenzymes

Question 12

This enzyme identify specific tissue damage and it is structurally different forms of the enzyme but it catalyzes the same reaction.

Answer 12

Isoenzymes

Question 13

This is produced by the prostate gland and the RBC

Answer 13

Acid Phosphatase

Question 14

What does H4 (LD1) represents and H3M (LD2) represents?

Answer 14

Heart, RBC and renal tubules

Question 15

What is the subunit and coefficient of Lungs, lymphocytes and spleen as a group?

Answer 15

H2M2

Question 16

What is the subunit and coefficient of Liver, and skeletal muscles?

Answer 16

HM3 (LD4) & M4 (LD5)

Question 17

What does the coefficient of the H & M subunits represent?

Answer 17

The rate of how the isoenzymes migrates in the electrophoresis.

Question 18

From 1-5 in the “LDH” coefficient. What number is the fastest?

Answer 18

1

Question 19

What are the characteristics of Isoenzymes?

Answer 19

1. Electrophoretic mobility
2. Mobility in Ion Exchange Resin
3. Response to inhibition
4. Relative substrate specificities

Question 20

Give an example of relative substrate specificities.

Answer 20

ACP (RCB) is less sensitive to a-naphthyl PO4

Question 21

This is the enzyme that is similar to the catalytic activity but are specie specific.

Answer 21

Heteroenzyme

Question 22

Give an example of Heteroenzyme.

Answer 22

LDH from Humans and Rabbits

Question 23

It is a genetically transmitted enzyme and is important in defining the biochemical characteristics of an individual.

Answer 23

Alloenzyme

Question 24

What is the main application of Alloenzyme?

Answer 24

Forensic and Genetic studies

Question 25

Under the Origin of Plasma Enzymes. Enumerate the Classification of enzymes in Blood.

Answer 25

Plasma-specific enzymes Non-plasma specific enzymes

Question 26

What organ secretes Plasma-Specific Enzyme?

Answer 26

Liver

Question 27

This enzyme exert their function in plasma and is also responsible in coagulation.

Answer 27

Plasma-specific enzyme.

Question 28

This enzymes has no specific functions in plasma. Thus, it lack activators or co-enzymes.

Answer 28

Non-plasma specific enzymes

Question 29

What are the two classes of Non-plasma specific enzymes

Answer 29

Enzymes of secretion Enzymes Associated with Cellular Metabolism

Question 30

This class of Non-plasma specific enzymes is fast to disappear in plasma and is secreted in plasma in high rates.

Answer 30

Enzymes of secretion

Question 31

Under Non-plasma specific enzymes, which class is referring to this? "It carries out their functions within the cells in which they are formed"

Answer 31

Enzymes Associated with Cellular Metabolism.

Question 32

Give an example of Enzymes associated with Cellular Metabolism.

Answer 32

Creatinine Kinase (Heart muscle)

Question 33

Enumerate the classification of Enzymes based on distribution.

Answer 33

A. UNILOCULAR ENZYME B. BILOCULAR ENZYME

Question 34

This classification of enzyme distribution based on distribution is found only in one location, particularly the cell sap.

Answer 34

Unilocular Enzyme

Question 35

This classification of enzyme distribution based on distribution is found in the mitochondria and cell sap.

Answer 35

Binocular Enzyme

Question 36

Enumerate the factors that affect binding of enzyme to substrate.

Answer 36

A. Energy B. Molecular Compatibility C. Space availability D. Specifity

Question 37

Under the factors that affect binding of enzyme to substrate. This initiates the reaction.

Answer 37

Energy

Question 38

Under the factors that affect binding of enzyme to substrate. This talks about the commonness between the substrate and enzyme.

Answer 38

Molecular Compatibility

Question 39

Under the factors that affect binding of enzyme to substrate. This talks about the # of enzyme/substrate that can be reacted.

Answer 39

Space Availability

Question 40

Under the factors that affect binding of enzyme to substrate. This talks about the enzyme acting on specific substrate.

Answer 40

Specificity

Question 41

What model best describes the E-S reaction.

Answer 41

Induced fit model

Question 42

Enumerate the factors that are INFLUENCING the ENZYMATIC REACTION

Answer 42

1. Time 2. Substrate concentration 3 Enzyme concentration 4. Temperature 5. pH 6. Activators 7. Inhibitors 8. Coenzyme concentration 9. Prosthetic group

Question 43

This is the rate of enzyme action.

Answer 43

Time

Question 44

Under time, what happens if the catalytic activity of an enzyme on the substrate is fast?

Answer 44

Shorter Reaction Time

Question 45

What relationship should a substrate concentration supposed to have?

Answer 45

Direct relationship

Question 46

This shows the relationship of the reaction velocity to the substrate concentration. Therefore, it will change depending on the substrate/ enzymatic concentration.

Answer 46

Michaelis-Menten Curve

Question 47

What are the phases of order kinetics?

Answer 47

First order kinetics Zero order kinetics

Question 48

This phase of order kinetics' enzyme concentration is FIXED. However, the substrate concentration is varied.

Answer 48

First order kinetics

Question 49

The reaction of this phase is almost directly proportional to substrate concentration at low values.

Answer 49

First Order Kinetics

Question 50

What is being affected by substrate concentration?

Answer 50

First order kinetics

Question 51

This phase is when maximum velocity is reached, the rate of increase in velocity is "0". Also, the rate in unaffected by increased substrate concentration.

Answer 51

Zero order kinetics

Question 52

What phase is dependent on the enzyme concentration and will only change if the Enzyme concentration is increased/decreased.

Answer 52

Zero Order KInetics

Question 53

The enzyme concentration says that "An increase in enzyme concentration results to an increase in the catalytic activity" THerefore, what relationship does it have?

Answer 53

Direct relationship

Question 54

What temperature is considered to be favorable for enzyme activity?

Answer 54

30 -37'C or 37 - 40'C

Question 55

What term is used for the "favorable temp for enzyme activity"?

Answer 55

Optimum temperature

Question 56

This is the reaction rate is doubled for every 10'C increase.

Answer 56

Q10

Question 57

What temperature does enzyme undergoes inactivation?

Answer 57

50-60'C