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Flashcards in Assay Tests Deck (9)
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1

Southern Blot

Probe --> ssDNA
Target --> DNA
Use --> Finding specific genes

2

Northern Blot

Probe --> ssDNA
Target --> mRNA
Use --> Size and quantity of mRNA

3

Western Blot

Probe --> Ab
Target --> Protein
Use --> | HIV Ags | [Ag or Protein] |

4

PCR

Probe --> DNA Primer
Target --> Sequence to be amplified
Use --> | detection of microorganisms | cystic fibrosis |

5

RFLP

Probe --> ssDNA
Target --> DNA
Use --> | Forensics | paternity test |

6

VNTR

Probe --> Restriction Enzymes
Target --> Short tandem Repeats
Use --> | Huntington's | Fragile X | Friedrich Ataxia |

7

Recombinant Proteins

Probe --> Human DNA
Target --> Plasmid
Use --> | Insulin | Clotting factors | Growth factors

8

Indirect ELISA

Probe --> Ag
Target --> Ab
Use --> ?

9

Sandwich ELISA

Probe --> Ab
Target --> Ag
Use --> | HIV (flourescent or colored) |